ArticleBiophysical journal2004
Studies of chi(2)/chi(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 176 citations in OpenAlex.
- Double Stokes polarimetric microscopy for chiral fibrillar aggregates.Scientific reports · 2025Article
- Investigating cartilage-related diseases by polarization-resolved second harmonic generation (P-SHG) imaging.APL bioengineering · 2024Article
- Article
- Polarimetric second harmonic generation microscopy of partially oriented fibers II: Imaging study.Biophysical journal · 2023Article
- Polarimetric second-harmonic generation microscopy of partially oriented fibers I: Digital modeling.Biophysical journal · 2023Article
- Second harmonic generation microscopy: a powerful tool for bio-imaging.Biophysical reviews · 2023Review
- Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization.Lasers in medical science · 2021Article
- Harmonic optical tomography of nonlinear structures.Nature photonics · 2020Article
- Label-free characterization of collagen fibers in cancerous esophagus tissues using ratiometric nonlinear optical microscopy.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Identifying crossing collagen fibers in human corneal tissues using pSHG images.Biomedical optics express · 2019Article
- Comparison of Different Polarization Sensitive Second Harmonic Generation Imaging Techniques.Methods and protocols · 2019Article
- Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience.Frontiers in molecular biosciences · 2019Review
- Enabling second harmonic generation as a contrast mechanism for optical projection tomography (OPT) and scanning laser optical tomography (SLOT).Biomedical optics express · 2018Article
- μMAPPS: a novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructure.Scientific reports · 2017Article
- Changes of collagen ultrastructure in breast cancer tissue determined by second-harmonic generation double Stokes-Mueller polarimetric microscopy.Biomedical optics express · 2016Article
- Connection of Jones and Mueller Tensors in Second Harmonic Generation and Multi-Photon Fluorescence Measurements.The journal of physical chemistry. B · 2016Article
- Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration.Biomedical optics express · 2015Article
- Translational label-free nonlinear imaging biomarkers to classify the human corneal microstructure.Biomedical optics express · 2015Article
- Applications of second-harmonic generation imaging microscopy in ovarian and breast cancer.Perspectives in medicinal chemistry · 2015Article
- Fast monitoring of in-vivo conformational changes in myosin using single scan polarization-SHG microscopy.Biomedical optics express · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical second- and third-harmonic generations have attracted a lot of attention in the biomedical imaging research field recently due to their intrinsic sectioning ability and noninvasiveness. Combined with near-infrared excitation sources, their deep-penetration ability makes these imaging modalities suitable for tissue characterization. In this article, we demonstrate a polarization harmonics optical microscopy, or P-HOM, to study the nonlinear optical anisotropy of the nanometer-scaled myosin and actin filaments inside myofibrils. By using tight focusing we can avoid the phase-matching condition due to micron-scaled, high-order structures in skeletal muscle fibers, and obtain the submicron-scaled polarization dependencies of second/third-harmonic generation intensities on the inclination angle between the long axes of the filaments and the polarization direction of the linear polarized fundamental excitation laser light. From these dependencies, detailed information on the tensor elements of the second/third-order nonlinear susceptibilities contributed from the myosin/actin filaments inside myofibrils can thus be analyzed and obtained, reflecting the detailed arrangements and structures of the constructing biomolecules. By acquiring a whole, nonlinearly sectioned image with a submicron spatial resolution, we can also compare the polarization dependency and calculate the nonlinear susceptibilities over a large area of the tissue at the same time-which not only provides statistical information but will be especially useful with complex specimen geometry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.