ArticleBiophysical journal2004
Cholera toxin assault on lipid monolayers containing ganglioside GM1.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 61 citations in OpenAlex.
- Recent Discovery of Diverse Prophages Located in Genomes ofFoods (Basel, Switzerland) · 2025Review
- Advancements in Engineering Planar Model Cell Membranes: Current Techniques, Applications, and Future Perspectives.Nanomaterials (Basel, Switzerland) · 2024Review
- Label-free quantification of protein binding to lipid vesicles using transparent waveguide evanescent-field scattering microscopy with liquid control.Biomedical optics express · 2023Article
- Uptake and intracellular fate of cholera toxin subunit b-modified mesoporous silica nanoparticle-supported lipid bilayers (aka protocells) in motoneurons.Nanomedicine : nanotechnology, biology, and medicine · 2018Article
- Interaction Between Luteinizing Hormone-Releasing Hormone and GM1-Doped Cholesterol/Sphingomyelin Vesicles: A Spectroscopic Study.The Journal of membrane biology · 2017Article
- A novel approach for targeted delivery to motoneurons using cholera toxin-B modified protocells.Journal of neuroscience methods · 2016Article
- Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species.Biomicrofluidics · 2014Article
- Ganglioside embedded in reconstituted lipoprotein binds cholera toxin with elevated affinity.Journal of lipid research · 2010Article
- Ordering transitions in micrometer-thick films of nematic liquid crystals driven by self-assembly of ganglioside GM1.Journal of colloid and interface science · 2009Article
- The interaction of beta-amyloid protein with cellular membranes stimulates its own production.Biochimica et biophysica acta · 2009Article
- Integration of ganglioside GT1b receptor into DPPE and DPPC phospholipid monolayers: an X-ray reflectivity and grazing-incidence diffraction study.Biophysical journal · 2008Article
- Lck-dependent Fyn activation requires C terminus-dependent targeting of kinase-active Lck to lipid rafts.The Journal of biological chemistry · 2008Article
- The small-molecule iron transport inhibitor ferristatin/NSC306711 promotes degradation of the transferrin receptor.Chemistry & biology · 2008Article
- Asymmetric structural features in single supported lipid bilayers containing cholesterol and GM1 resolved with synchrotron X-Ray reflectivity.Biophysical journal · 2008Article
- Part I: an x-ray scattering study of cholera toxin penetration and induced phase transformations in lipid membranes.Biophysical journal · 2008Article
- Characterization of physical properties of supported phospholipid membranes using imaging ellipsometry at optical wavelengths.Biophysical journal · 2007Article
- Rearrangement of lipid ordered phases upon protein adsorption due to multiple site binding.Physical review letters · 2006Article
- Local mobility in lipid domains of supported bilayers characterized by atomic force microscopy and fluorescence correlation spectroscopy.Biophysical journal · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many bacterial toxins bind to and gain entrance to target cells through specific interactions with membrane components. Using neutron reflectivity, we have characterized the structure of mixed DPPE:GM(1) lipid monolayers before and during the binding of cholera toxin (CTAB(5)) or its B-subunit (CTB(5)). Structural parameters such as the density and thickness of the lipid layer, extension of the GM(1) oligosaccharide headgroup, and orientation and position of the protein upon binding are reported. The density of the lipid layer was found to decrease slightly upon protein binding. However, the A-subunit of the whole toxin is clearly located below the B-pentameric ring, away from the monolayer, and does not penetrate into the lipid layer before enzymatic cleavage. Using Monte Carlo simulations, the observed monolayer expansion was found to be consistent with geometrical constraints imposed on DPPE by multivalent binding of GM(1) by the toxin. Our findings suggest that the mechanism of membrane translocation by the protein may be aided by alterations in lipid packing.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.