ArticleBiophysical journal2004
Time-resolved charge translocation by sarcoplasmic reticulum Ca-ATPase measured on a solid supported membrane.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Sarcolipin is a Conformation-Dependent Regulator of the Sarcoplasmic Reticulum Calcium Pump SERCA.The Journal of membrane biology · 2026Article
- Structural Basis for the Function of the C-Terminal Proton Release Pathway in the Calcium Pump.International journal of molecular sciences · 2021Article
- Multiscale Simulation Reveals Passive Proton Transport Through SERCA on the Microsecond Timescale.Biophysical journal · 2020Article
- Functional Characterization of SLC Transporters Using Solid Supported Membranes.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Phosphatidylserine flipping by the P4-ATPase ATP8A2 is electrogenic.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Mechanism of the E2 to E1 transition in CaProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Conformational memory in the association of the transmembrane protein phospholamban with the sarcoplasmic reticulum calcium pump SERCA.The Journal of biological chemistry · 2017Article
- Hofmeister effect of anions on calcium translocation by sarcoplasmic reticulum Ca(2+)-ATPase.Scientific reports · 2015Article
- Ca(2+)/H (+) exchange, lumenal Ca(2+) release and Ca (2+)/ATP coupling ratios in the sarcoplasmic reticulum ATPase.Journal of cell communication and signaling · 2014Article
- Istaroxime stimulates SERCA2a and accelerates calcium cycling in heart failure by relieving phospholamban inhibition.British journal of pharmacology · 2013Article
- Distinctive features of catalytic and transport mechanisms in mammalian sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) and Cu+ (ATP7A/B) ATPases.The Journal of biological chemistry · 2012Article
- Assaying the proton transport and regulation of UCP1 using solid supported membranes.European biophysics journal : EBJ · 2012Article
- High-yield heterologous expression of wild type and mutant Ca(2+) ATPase: Characterization of Ca(2+) binding sites by charge transfer.Journal of molecular biology · 2009Article
- Effect of clotrimazole on the pump cycle of the Na,K-ATPase.Biophysical journal · 2008Article
- Effects of high-affinity inhibitors on partial reactions, charge movements, and conformational States of the Ca2+ transport ATPase (sarco-endoplasmic reticulum Ca2+ ATPase).Molecular pharmacology · 2008Article
- Side-chain protonation and mobility in the sarcoplasmic reticulum Ca2+-ATPase: implications for proton countertransport and Ca2+ release.Biophysical journal · 2007Article
- Structural role of countertransport revealed in Ca(2+) pump crystal structure in the absence of Ca(2+).Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sarcoplasmic reticulum vesicles were adsorbed on an octadecanethiol/phosphatidylcholine mixed bilayer anchored to a gold electrode, and the Ca-ATPase contained in the vesicles was activated by ATP concentration jumps both in the absence and in the presence of K(+) ions and at different pH values. Ca(2+) concentration jumps in the absence of ATP were also carried out. The resulting capacitive current transients were analyzed together with the charge under the transients. The relaxation time constants of the current transients were interpreted on the basis of an equivalent circuit. The current transient after ATP concentration jumps and the charge after Ca(2+) concentration jumps in the absence of ATP exhibit almost the same dependence upon the Ca(2+) concentration, with a half-saturating value of approximately 1.5 microM. The pH dependence of the charge after Ca(2+) translocation demonstrates the occurrence of one H(+) per one Ca(2+) countertransport at pH 7 by direct charge-transfer measurements. The presence of K(+) decreases the magnitude of the current transients without altering their shape; this decrease is explained by K(+) binding to the cytoplasmic side of the pump in the E(1) conformation and being released to the same side during the E(1)-E(2) transition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.