ArticleBiophysical journal2004
Functional properties of the Drosophila melanogaster inositol 1,4,5-trisphosphate receptor mutants.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- Visual input drives diverse ER calcium signals in neuronsbioRxiv : the preprint server for biology · 2025Article
- Transcriptomic Characterization of Phototransduction Genes of the Asian Citrus PsyllidInsects · 2024Article
- Functional determination of calcium-binding sites required for the activation of inositol 1,4,5-trisphosphate receptors.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- From spikes to intercellular waves: Tuning intercellular calcium signaling dynamics modulates organ size control.PLoS computational biology · 2021Article
- A Comparative Perspective on Functionally-Related, Intracellular Calcium Channels: The Insect Ryanodine and Inositol 1,4,5-Trisphosphate Receptors.Biomolecules · 2021Review
- Distinct Dopamine Receptor Pathways Underlie the Temporal Sensitivity of Associative Learning.Cell · 2019Article
- Article
- IPDevelopment (Cambridge, England) · 2017Article
- Spontaneous CaFrontiers in molecular neuroscience · 2017Article
- Spastin, atlastin, and ER relocalization are involved in axon but not dendrite regeneration.Molecular biology of the cell · 2016Article
- Mutant IP3 receptors attenuate store-operated Ca2+ entry by destabilizing STIM-Orai interactions in Drosophila neurons.Journal of cell science · 2016Article
- Functional cooperation between the IP3 receptor and phospholipase C secures the high sensitivity to light of Drosophila photoreceptors in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Bilayer measurement of endoplasmic reticulum Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- A genetic RNAi screen for IP₃/Ca²⁺ coupled GPCRs in Drosophila identifies the PdfR as a regulator of insect flight.PLoS genetics · 2013Article
- Linking structure to function: Recent lessons from inositol 1,4,5-trisphosphate receptor mutagenesis.Cell calcium · 2010Review
- Inositol 1,4,5- trisphosphate receptor function in Drosophila insulin producing cells.PloS one · 2009Article
- Intracellular Ca2+ signaling and store-operated Ca2+ entry are required in Drosophila neurons for flight.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Surface accessibility and conformational changes in the N-terminal domain of type I inositol trisphosphate receptors: studies using cysteine substitution mutagenesis.The Journal of biological chemistry · 2009Article
- cAMP potentiates InsP3-induced Ca2+ release from the endoplasmic reticulum in blowfly salivary glands.BMC physiology · 2008Article
- Homeostasis of glutamate neurotransmission is altered in Drosophila Inositol 1,4,5-trisphosphate receptor mutants.Invertebrate neuroscience : IN · 2007Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The inositol (1,4,5)-trisphosphate receptor (InsP(3)R) is an intracellular calcium (Ca(2+)) release channel that plays a crucial role in cell signaling. In Drosophila melanogaster a single InsP(3)R gene (itpr) encodes a protein (DmInsP(3)R) that is approximately 60% conserved with mammalian InsP(3)Rs. A number of itpr mutant alleles have been identified in genetic screens and studied for their effect on development and physiology. However, the functional properties of wild-type or mutant DmInsP(3)Rs have never been described. Here we use the planar lipid bilayer reconstitution technique to describe single-channel properties of embryonic and adult head DmInsP(3)R splice variants. The three mutants chosen in this study reside in each of the three structural domains of the DmInsP(3)R-the amino-terminal ligand binding domain (ug3), the middle-coupling domain (wc703), and the channel-forming region (ka901). We discovered that 1), the major functional properties of DmInsP(3)R (conductance, gating, and sensitivity to InsP(3) and Ca(2+)) are remarkably conserved with the mammalian InsP(3)R1; 2), single-channel conductance of the adult head DmInsP(3)R isoform is 89 pS and the embryonic DmInsP(3)R isoform is 70 pS; 3), ug3 mutation affects sensitivity of the DmInsP(3)Rs to activation by InsP(3), but not their InsP(3)-binding properties; 4), wc703 channels have increased sensitivity to modulation by Ca(2+); and 5), homomeric ka901 channels are not functional. We correlated the results obtained in planar lipid bilayer experiments with measurements of InsP(3)-induced Ca(2+) fluxes in microsomes isolated from wild-type and heterozygous itpr mutants. Our study validates the use of D. melanogaster as an appropriate model for InsP(3)R structure-function studies and provides novel insights into the fundamental mechanisms of the InsP(3)R function.
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