ArticleProceedings of the National Academy of Sciences of the United States of America1992
Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 208 citations in OpenAlex.
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- Effects of tamoxifen inducible MerCreMer on gene expression in cardiac myocytes in mice.The journal of cardiovascular aging · 2022Article
- A CRISPR-Cas9, Cre-The CRISPR journal · 2020Article
- A most formidable arsenal: genetic technologies for building a better mouse.Genes & development · 2020Review
- A Structured Workflow for Mapping Human Sin3 Histone Deacetylase Complex Interactions Using Halo-MudPIT Affinity-Purification Mass Spectrometry.Molecular & cellular proteomics : MCP · 2018Article
- Low- and High-LET Ionizing Radiation Induces Delayed Homologous Recombination that Persists for Two Weeks before Resolving.Radiation research · 2017Article
- Transcription Activator-Like Effector Nucleases (TALEN)-Mediated Targeted DNA Insertion in Potato Plants.Frontiers in plant science · 2016Article
- Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals.Cellular and molecular life sciences : CMLS · 2015Review
- A pathway from chromosome transfer to engineering resulting in human and mouse artificial chromosomes for a variety of applications to bio-medical challenges.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Accurate comparison of antibody expression levels by reproducible transgene targeting in engineered recombination-competent CHO cells.Applied microbiology and biotechnology · 2014Article
- Expanding the scope of site-specific recombinases for genetic and metabolic engineering.Biotechnology and bioengineering · 2014Review
- Rapid genetic modification of mouse embryonic stem cells by Inducible Cassette Exchange recombination.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells.Journal of industrial microbiology & biotechnology · 2013Review
- Inducible cassette exchange: a rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells.Stem cells (Dayton, Ohio) · 2011Article
- Generation of a Slc39a8 hypomorph mouse: markedly decreased ZIP8 Zn²⁺/(HCO₃⁻)₂ transporter expression.Biochemical and biophysical research communications · 2011Article
- Conditional gene targeting: dissecting the cellular mechanisms of retinal degenerations.Journal of ophthalmology · 2011Article
- A biobrick library for cloning custom eukaryotic plasmids.PloS one · 2011Article
- Article
- Applications of the site-specific recombinase Cre to the study of genomic imprinting.Briefings in functional genomics · 2010Review
- Comparative analysis of right element mutant lox sites on recombination efficiency in embryonic stem cells.BMC biotechnology · 2010Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stable transformants of mammalian cells from gene transfer often show extreme variability in expression of the introduced transgene. This occurs from the highly variable number of copies integrated into the genome and from position effects on gene expression due to random integration. We have eliminated both of these constraints on predictable gene expression by use of a lox recombination vector. The positive selection vector system is designed to directly select Cre-mediated DNA integration at a lox target previously placed into the genome of cultured mammalian cells. Proper targeting activates expression of a defective lox-neomycin phosphotransferase (neo) fusion gene target. With CHO cell lines containing this target, almost all of the selected transformants (54 of 56 independent G418-resistant colonies) were simple single-copy integrants of the targeting DNA. To monitor gene expression at a single chromosomal site, we used a beta-actin promoter-lacZ reporter construct. Independent G418-resistant colonies from site-specific integration of the reporter gene all showed nearly identical levels of beta-galactosidase activity when the reporter construct integrated at a particular chromosomal position. The same construct integrated at a second chromosomal position exhibited a slightly different level of activity, characteristic of that second position. These results show that Cre-mediated site-specific integration can facilitate the construction of isogenic cell lines and thereby permit reproducible gene expression in stably transformed cell lines.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.