ArticleNature structural & molecular biology2004
DNA transport into Bacillus subtilis requires proton motive force to generate large molecular forces.
Article in Nature structural & molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 106 citations in OpenAlex.
- Molecular interplay between ComEC domains allows for selective degradation of the non-translocating strand during natural transformation.Nucleic acids research · 2025Article
- Uptake of environmental DNA in Bacillus subtilis occurs all over the cell surface through a dynamic pilus structure.PLoS genetics · 2023Article
- Cell-to-cell natural transformation in Bacillus subtilis facilitates large scale of genomic exchanges and the transfer of long continuous DNA regions.Nucleic acids research · 2023Article
- Factors and Mechanisms Influencing Conjugation In Vivo in the Gastrointestinal Tract Environment: A Review.International journal of molecular sciences · 2023Review
- Single-Molecule Dynamics of DNA Receptor ComEA, Membrane Permease ComEC, and Taken-Up DNA in Competent Bacillus subtilis Cells.Journal of bacteriology · 2022Article
- Natural Transformation Protein ComFA Exhibits Single-Stranded DNA Translocase Activity.Journal of bacteriology · 2022Article
- "Take It or Leave It"-Factors Regulating Competence Development and DNA Uptake inInternational journal of molecular sciences · 2021Article
- The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation.Molecular microbiology · 2021Article
- Article
- DNA Uptake by Type IV Filaments.Frontiers in molecular biosciences · 2019Review
- Article
- Kinetics of DNA uptake during transformation provide evidence for a translocation ratchet mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Impact of donor-recipient phylogenetic distance on bacterial genome transplantation.Nucleic acids research · 2016Article
- Single-Stranded DNA Uptake during Gonococcal Transformation.Journal of bacteriology · 2016Article
- A Comparative Structure/Function Analysis of Two Type IV Pilin DNA Receptors Defines a Novel Mode of DNA Binding.Structure (London, England : 1993) · 2016Article
- Natural transformation occurs independently of the essential actin-like MreB cytoskeleton in Legionella pneumophila.Scientific reports · 2015Article
- Gene Transfer Efficiency in Gonococcal Biofilms: Role of Biofilm Age, Architecture, and Pilin Antigenic Variation.Journal of bacteriology · 2015Article
- Uptake of extracellular DNA: competence induced pili in natural transformation of Streptococcus pneumoniae.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- Conserved Streptococcus pneumoniae spirosomes suggest a single type of transformation pilus in competence.PLoS pathogens · 2015Article
- Mobile DNA in the pathogenicMicrobiology spectrum · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Bacteria can acquire genetic diversity, including antibiotic resistance and virulence traits, by horizontal gene transfer. In particular, many bacteria are naturally competent for uptake of naked DNA from the environment in a process called transformation. Here, we used optical tweezers to demonstrate that the DNA transport machinery in Bacillus subtilis is a force-generating motor. Single DNA molecules were processively transported in a linear fashion without observable pausing events. Uncouplers inhibited DNA uptake immediately, suggesting that the transmembrane proton motive force is needed for DNA translocation. We found an uptake rate of 80 +/- 10 bp s(-1) that was force-independent at external forces <40 pN, indicating that a powerful molecular machine supports DNA transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.