ArticleProceedings of the National Academy of Sciences of the United States of America2004
Interplay between Drosophila Bloom's syndrome helicase and Ku autoantigen during nonhomologous end joining repair of P element-induced DNA breaks.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 36 citations in OpenAlex.
- Homologous recombination is essential for DNA damage-induced regeneration of Drosophila testis germline stem cells.Development (Cambridge, England) · 2026Article
- Mechanism and regulation of P element transposition.Open biology · 2020Review
- Article
- Mosaic Analysis inGenetics · 2018Review
- Review
- Drosophila IRBP bZIP heterodimer binds P-element DNA and affects hybrid dysgenesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- P Transposable Elements in Drosophila and other Eukaryotic Organisms.Microbiology spectrum · 2015Review
- Genomic landscape of human, bat, and ex vivo DNA transposon integrations.Molecular biology and evolution · 2014Article
- Females and males contribute in opposite ways to the evolution of gene order in Drosophila.PloS one · 2013Article
- Article
- Article
- Article
- DNA strand displacement, strand annealing and strand swapping by the Drosophila Bloom's syndrome helicase.Nucleic acids research · 2007Article
- Template disruptions and failure of double Holliday junction dissolution during double-strand break repair in Drosophila BLM mutants.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Mechanisms of RecQ helicases in pathways of DNA metabolism and maintenance of genomic stability.The Biochemical journal · 2006Review
- Topoisomerase IIIalpha and Bloom's helicase can resolve a mobile double Holliday junction substrate through convergent branch migration.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- DNA damage and L1 retrotransposition.Journal of biomedicine & biotechnology · 2006Article
- Formation of deletions during double-strand break repair in Drosophila DmBlm mutants occurs after strand invasion.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
P transposable elements in Drosophila are mobilized via a cut-and-paste mechanism. The broken DNA ends generated during transposition can be repaired via the homology-directed synthesis-dependent strand annealing or by nonhomologous end joining (NHEJ). Genetic studies have demonstrated an interaction between the gene (mus309, for mutagen-sensitive) encoding the Drosophila Bloom's syndrome helicase homolog (DmBLM) and the Ku70 gene, which is involved in NHEJ. We have used RNA interference (RNAi) to knock down expression of DmBLM and one or both of the Drosophila Ku subunits, DmKu70 or DmKu80. Our results show that upon reduction of DmKu, an increase in small deletions (1-49 bp) and large deletions (>/=50 bp) flanking the site of P element-induced breaks is observed, and a reduction in large deletions at these sites is found upon reduction of DmBLM. Moreover, double RNAi of DmKu and DmBLM results in an increase in small deletions characteristic of the DmKu RNAi and also partially suppresses the reduction in repair efficiency observed with DmKu RNAi. These results suggest that there are DNA double-strand break recognition and/or processing events involving DmKu and DmBLM that, when eliminated by RNAi, lead to deletions. Finally, these results raise the possibility that, unlike the situation in mammals, where BLM appears to function exclusively in the homologous repair pathway, in Drosophila, DmBLM may be directly involved in, or at least influence the double-strand break recognition that leads to the NHEJ repair pathway.
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