Evidence map›Paper›PMID 15184482›Full record

ArticleJournal of clinical microbiology2004

Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens.

Santanu Chattopadhyay, Rajashree Patra, T Ramamurthy, Abhijit Chowdhury, Amal Santra, G K Dhali, S K Bhattacharya, Douglas E Berg, G Balakrish Nair, Asish K Mukhopadhyay

Open access · greenAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 1 synthesis or guideline pooled it, 122 citations in OpenAlex.

  1. Non-invasive diagnostic tests for Helicobacter pylori infection.The Cochrane database of systematic reviews · 2018
    Pooled it
  2. Journal of medical microbiology · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Helicobacter pylori cagA, vacA, and iceA genotypes and clinical outcomes: a cross-sectional study in central Vietnam.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024
    Article
  8. Article
  9. Article
  10. Article
  11. Pathogens (Basel, Switzerland) · 2023
    Article
  12. Article
  13. Article
  14. LowFrontiers in microbiology · 2021
    Article
  15. Article
  16. Genotyping ofInternational journal of microbiology · 2021
    Article
  17. A Mouse Model of Helicobacter pylori Infection.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
  18. Analysis of Correlation between the Seven ImportantGastroenterology research and practice · 2020
    Article
  19. Concurrent detection ofIranian journal of microbiology · 2019
    Article
  20. DifferentialPathogens (Basel, Switzerland) · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Santanu ChattopadhyayNational Institute of Cholera and Enteric Diseases, Calcutta 700010, India.
Rajashree Patra
T Ramamurthy
Abhijit Chowdhury
Amal Santra
G K Dhali
S K Bhattacharya
Douglas E Berg
G Balakrish Nair
Asish K Mukhopadhyay
National Institute of Cholera and Enteric Diseases · INInstitute of Post Graduate Medical Education and Research · INInternational Centre for Diarrhoeal Disease Research · BDWashington University in St. Louis · US

Funding

H.PYLORI POPULATION GENETICS AND GENOME EVOLUTIONR01DK063041 · NIDDK · WASHINGTON UNIVERSITY · PI BERG, DOUGLAS EUGENE · 2003 to 2007
$1.6M
H. PYLORI INFECTION AND GI DISEASE IN ALASKA NATIVESR01DK053727 · NIDDK · WASHINGTON UNIVERSITY · PI BERG, DOUGLAS EUGENE · 1999 to 2003
$1.3M
DRUG RESISTANCE AND GENE TRANSFER IN H PYLORIR01AI038166 · NIAID · WASHINGTON UNIVERSITY · PI BERG, DOUGLAS EUGENE · 1995 to 2003
$1.1M
NIAID NIH HHS AI 38166NIDDK NIH HHS DK 53727NIDDK NIH HHS DK 63041NIDDK NIH HHS R01 DK063041
6 · The paper itself

Abstract

We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries.

Indexed as

Antigens, BacterialBacterial ProteinsBiopsyGenotypeHelicobacter InfectionsHelicobacter pyloriHumansPolymerase Chain ReactionAntigens, BacterialBacterial ProteinscagA protein, Helicobacter pyloriVacA protein, Helicobacter pylori

Identifiers

PMID15184482
PMCPMC427847
OpenAlexW2136287933

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.