ArticleJournal of clinical microbiology2004
Use of the hupB gene encoding a histone-like protein of Mycobacterium tuberculosis as a target for detection and differentiation of M. tuberculosis and M. bovis.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Accurate Identification of Closely RelatedFrontiers in cellular and infection microbiology · 2021Article
- Molecular detection of M. tuberculosis and M. bovis and hematological and biochemical analyses in agricultural sprayers exposed to pesticides: A cross-sectional study in Punjab, Pakistan during 2014-2016.Journal of exposure science & environmental epidemiology · 2017Article
- Expression of antimicrobial drug tolerance by attached communities of Mycobacterium tuberculosis.Pathogens and disease · 2014Article
- Detection of IS6110 and HupB gene sequences of Mycobacterium tuberculosis and bovis in the aortic tissue of patients with Takayasu's arteritis.BMC infectious diseases · 2012Article
- Pyrosequencing assay for rapid identification of Mycobacterium tuberculosis complex species.BMC research notes · 2011Article
- PCR-Based Evaluation of Tuberculous Endometritis in Infertile Women of North India.Journal of reproduction & infertility · 2011Article
- DNA clasping by mycobacterial HU: the C-terminal region of HupB mediates increased specificity of DNA binding.PloS one · 2010Article
- Association of tuberculous endometritis with infertility and other gynecological complaints of women in India.Journal of clinical microbiology · 2008Article
- A single-step sequencing method for the identification of Mycobacterium tuberculosis complex species.PLoS neglected tropical diseases · 2008Article
- The mycobacterial DNA-binding protein 1 (MDP1) from Mycobacterium bovis BCG influences various growth characteristics.BMC microbiology · 2008Article
- Identification and characterization of a major cell wall-associated iron-regulated envelope protein (Irep-28) in Mycobacterium tuberculosis.Clinical and vaccine immunology : CVI · 2006Article
- Occurrence of overlooked zoonotic tuberculosis: detection of Mycobacterium bovis in human cerebrospinal fluid.Journal of clinical microbiology · 2006Article
- Direct detection and identification of Mycobacterium tuberculosis and Mycobacterium bovis in bovine samples by a novel nested PCR assay: correlation with conventional techniques.Journal of clinical microbiology · 2005Article
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7 authors.
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Abstract
The gene for histone-like protein (hupB [Rv2986c]) of Mycobacterium tuberculosis has been identified as a singular target which allows differentiation of two closely related mycobacterial species, namely, M. tuberculosis and M. bovis of the MTB complex, by a PCR assay. The N and S primer-generated PCR amplicons differed in M. tuberculosis and M. bovis; these amplicons were determined to be 645 and 618 bp, respectively. This difference was localized to the C-terminal part of the gene by using primers M and S. The C-terminal PCR amplicons of M. tuberculosis and M. bovis were determined to be 318 and 291 bp, respectively. The differences in the C-terminal portion of the gene were confirmed by restriction fragment length polymorphism analysis and sequencing. Sequence analysis indicated that in M. bovis there was a deletion of 27 bp (9 amino acids) in frame after codon 128 in the C-terminal part of the hupB gene. In the present study 104 mycobacterial strains and 11 nonmycobacterial species were analyzed for hupB gene sequences. Of the 104 mycobacterial strains included, 62 belonged to the MTB complex and 42 were non-MTB complex strains and species. Neither the hupB gene-specific primers (N and S) nor the C-terminal primers (M and S) amplify DNA from any other mycobacteria, making the assay suitable for distinguishing members of the MTB complex from other mycobacterial species, as well as for differentiating between members of the MTB complex, namely, M. tuberculosis and M. bovis.
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