ArticleJournal of clinical microbiology2004
Characterization of novel coding sequences specific to Mycobacterium avium subsp. paratuberculosis: implications for diagnosis of Johne's Disease.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 65 citations in OpenAlex.
- Complete Genome Sequence of OvineMicroorganisms · 2020Article
- Diagnostic Sequences That DistinguishFrontiers in veterinary science · 2020Article
- Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays.PloS one · 2017Article
- Identification of sero-reactive antigens for the early diagnosis of Johne's disease in cattle.PloS one · 2017Article
- Comparative genomics between human and animal associated subspecies of the Mycobacterium avium complex: a basis for pathogenicity.BMC genomics · 2015Article
- Disruption of Mycobacterium avium subsp. paratuberculosis-specific genes impairs in vivo fitness.BMC genomics · 2014Article
- Genomic variations associated with attenuation in Mycobacterium avium subsp. paratuberculosis vaccine strains.BMC microbiology · 2013Article
- Transabdominal ultrasonographic findings in goats with paratuberculosis.The Canadian veterinary journal = La revue veterinaire canadienne · 2012Article
- Clinical, ultrasonographic, and pathologic findings in 70 camels (Camelus dromedarius) with Johne's disease.The Canadian veterinary journal = La revue veterinaire canadienne · 2012Article
- Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics.PLoS neglected tropical diseases · 2010Article
- Genomic comparison of PE and PPE genes in the Mycobacterium avium complex.Journal of clinical microbiology · 2009Article
- Immunogenicity of proteome-determined Mycobacterium avium subsp. paratuberculosis-specific proteins in sheep with paratuberculosis.Clinical and vaccine immunology : CVI · 2008Article
- New triplex real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in bovine feces.Applied and environmental microbiology · 2008Article
- Comparative genomic analysis of Mycobacterium avium subspecies obtained from multiple host species.BMC genomics · 2008Article
- Profiling bovine antibody responses to Mycobacterium avium subsp. paratuberculosis infection by using protein arrays.Infection and immunity · 2008Article
- Early antibody response against Mycobacterium avium subspecies paratuberculosis antigens in subclinical cattle.Proteome science · 2008Article
- Defining the stressome of Mycobacterium avium subsp. paratuberculosis in vitro and in naturally infected cows.Journal of bacteriology · 2007Article
- Mycobacterium avium in the postgenomic era.Clinical microbiology reviews · 2007Review
- Production and characterization of monoclonal antibodies against a major membrane protein of Mycobacterium avium subsp. paratuberculosis.Clinical and vaccine immunology : CVI · 2007Article
- Whole-genome plasticity among Mycobacterium avium subspecies: insights from comparative genomic hybridizations.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's Disease, an economically important intestinal ailment of ruminants. Due to the considerable genetic and serologic cross-reactivity with closely related and ubiquitous members of the M. avium complex, a species-specific method for the serological diagnosis of Johne's disease is unavailable. Computational and PCR-based analysis of the complete genome sequence of M. avium subsp. paratuberculosis led to the identification of 13 open reading frames with no identifiable homologs. One of these sequences is a putative insertion element present in six copies on the M. avium subsp. paratuberculosis genome. These novel M. avium subsp. paratuberculosis genes were cloned into Escherichia coli expression vectors, and nine were successfully expressed as recombinant fusion proteins. Five of these proteins were purified in sufficient amounts to allow immunoblot analyses of their reactivity with sera from naturally infected cattle as well as mice and rabbits exposed to M. avium subsp. paratuberculosis. Fusion proteins representing MAP0862, MAP3732c, and MAP2963c were recognized by nearly all of the sera tested, including those from cattle in the clinical stages of disease. Notably, further analysis of the protein encoded by MAP0862 showed that it reacted with sera from additional infected cattle but not with sera from uninfected control animals. The fusion product of MAP0860c did not react with any of the sera tested, while the remaining four proteins were variably recognized by sera from M. avium subsp. paratuberculosis-infected cattle. Collectively, the results of this study demonstrate the utility of genomic data to identify potential diagnostic sequences.
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