ArticleJournal of clinical microbiology2004
Real-time PCR assay using fine-needle aspirates and tissue biopsy specimens for rapid diagnosis of mycobacterial lymphadenitis in children.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 144 citations in OpenAlex.
- Mycobacterium haemophilum and lymphadenitis in children.Emerging infectious diseases · 2005Trial
- Nontuberculous Mycobacteria Infections in Children: A Clinical Overview of Diagnosis and Management.Microorganisms · 2026Review
- Aircraft lavatory wastewater surveillance for movement of antimicrobial resistance genes: a proof-of-concept study.Microbiology spectrum · 2025Article
- Meta-Transcriptomic Response to Copper Corrosion in Drinking Water Biofilms.Microorganisms · 2025Article
- A 28-Year Multicenter Cohort Study of Nontuberculous Mycobacterial Lymphadenitis in Children, Spain.Emerging infectious diseases · 2025Article
- Cure Rates for Tuberculous Cervical Lymphadenopathy after 6-month or 9-month Anti-tuberculous Therapy.Acta medica Philippina · 2024Article
- Manifestations of cutaneous mycobacterial infections in patients with inborn errors of IL-12/IL-23-IFNγ immunityEuropean journal of dermatology : EJD · 2022Article
- Molecular Diagnosis of Cat Scratch Disease: a 25-Year Retrospective Comparative Analysis of Various Clinical Specimens and Different PCR Assays.Microbiology spectrum · 2022Article
- Radiomics and machine learning for the diagnosis of pediatric cervical non-tuberculous mycobacterial lymphadenitis.Scientific reports · 2022Article
- Management of Infectious Lymphadenitis in Children.Children (Basel, Switzerland) · 2021Review
- Article
- Molecular strategy for the diagnosis of infectious lymphadenitis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Molecular Tests That Target the RTX Locus Do Not Distinguish between Kingella kingae and the Recently Described Kingella negevensis Species.Journal of clinical microbiology · 2017Article
- Acute Septic Arthritis of the Knee Caused byFrontiers in pediatrics · 2017Article
- Validation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System.Journal of clinical microbiology · 2016Article
- Article
- Detection of mycobacteria, Mycobacterium avium subspecies, and Mycobacterium tuberculosis complex by a novel tetraplex real-time PCR assay.Journal of clinical microbiology · 2015Article
- Mycobacterium avium Subspecies paratuberculosis: Human Exposure through Environmental and Domestic Aerosols.Pathogens (Basel, Switzerland) · 2014Article
- Bacterial lymphadenitis at a major referral hospital in France from 2008 to 2012.Journal of clinical microbiology · 2014Article
- Environmental distribution and seasonal prevalence of Mycobacterium ulcerans in Southern Louisiana.Applied and environmental microbiology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time PCR assay was developed to diagnose and identify the causative agents of suspected mycobacterial lymphadenitis. Primers and probes for the real-time PCR were designed on the basis of the internal transcribed spacer sequence, enabling the recognition of the genus Mycobacterium and the species Mycobacterium avium and M. tuberculosis. The detection limit for the assay was established at 1,100 CFU/ml of pus, and the specificity tests showed no false-positive reaction with other mycobacterial species and other pathogens causing lymphadenitis. From 67 children with suspected mycobacterial lymphadenitis based on a positive mycobacterial skin test, 102 samples (58 fine-needle aspirates [FNA] and 44 tissue specimens) were obtained. The real-time PCR assay detected a mycobacterial infection in 48 patients (71.6%), whereas auramine staining and culturing were positive for 31 (46.3%) and 28 (41.8%) of the patients. The addition of the real-time PCR assay to conventional diagnostic tests resulted in the recognition of 13 more patients with mycobacterial disease. These results indicate that the real-time PCR is more sensitive than conventional staining and culturing techniques (P = 0.006). The M. avium-specific real-time PCR was positive for 38 patients, and the M. tuberculosis-specific real-time PCR was positive for 1 patient. Analysis of 27 patients from whom FNA and tissue biopsy specimens were collected revealed significantly more positive real-time PCR results for FNA than for tissue biopsy specimens (P = 0.003). Samples from an age-matched control group of 50 patients with PCR-proven cat scratch disease were all found to be negative by the real-time PCR. We conclude that this real-time PCR assay with a sensitivity of 72% for patients with lymphadenitis and a specificity of 100% for the detection of atypical mycobacteria can provide excellent support for clinical decision making in children with lymphadenitis.
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