ArticleJournal of clinical microbiology2004
Evaluation and comparison of two commercial enzyme-linked immunosorbent assay kits for detection of antigenically diverse human noroviruses in stool samples.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 119 citations in OpenAlex.
- Drivers of the paradigm shift in norovirus diagnostics: technological innovation, contextual demands, and collaborative synergy.Virology journal · 2026Review
- A study on the occurrence of human enteric viruses in salad vegetables and seafood and associated health risks for consumers in Mauritius.Italian journal of food safety · 2023Article
- Norovirus detection in water samples at the level of single virus copies per microliter using a smartphone-based fluorescence microscope.Nature protocols · 2021Article
- Performance evaluation of Automated Fluorescent Immunoassay System ROTA and NORO for detection of rotavirus and norovirus: A comparative study of assay performance with RIDASCREENJournal of clinical laboratory analysis · 2021Article
- Review
- Identification and Characterization of Single-Chain Antibodies that Specifically Bind GI Noroviruses.PloS one · 2017Article
- Nanobody binding to a conserved epitope promotes norovirus particle disassembly.Journal of virology · 2015Article
- Characterization of cross-reactive norovirus-specific monoclonal antibodies.Clinical and vaccine immunology : CVI · 2015Article
- Norovirus.Clinical microbiology reviews · 2015Review
- Microbiologic methods utilized in the MAL-ED cohort study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Article
- Persistent digestive disorders in the tropics: causative infectious pathogens and reference diagnostic tests.BMC infectious diseases · 2013Review
- Evaluation of the SD Bioline Norovirus rapid immunochromatography test using fecal specimens from Korean gastroenteritis patients.Journal of virological methods · 2012Article
- Bioluminescent enzyme immunoassay for the detection of norovirus capsid antigen.Clinical and vaccine immunology : CVI · 2012Article
- The dynamics of norovirus outbreak epidemics: recent insights.International journal of environmental research and public health · 2011Review
- Diagnostic accuracy and analytical sensitivity of IDEIA Norovirus assay for routine screening of human norovirus.Journal of clinical microbiology · 2010Article
- Apparently non-specific results found using a norovirus antigen immunoassay for fecal specimens from neonates.Journal of perinatology : official journal of the California Perinatal Association · 2008Article
- Epidemiological and virological investigation of a Norovirus outbreak in a resort in Puglia, Italy.BMC infectious diseases · 2007Article
- European multicenter evaluation of commercial enzyme immunoassays for detecting norovirus antigen in fecal samples.Clinical and vaccine immunology : CVI · 2007Article
- Porcine enteric caliciviruses: genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology.Vaccine · 2007Review
- Detection of norovirus antigens from recombinant virus-like particles and stool samples by a commercial norovirus enzyme-linked immunosorbent assay kit.Journal of clinical microbiology · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two recently commercialized enzyme-linked immunosorbent assay kits, the SRSV(II)-AD (Denka Seiken Co. Ltd., Tokyo, Japan) and IDEIA NLV (DakoCytomation Ltd., Ely, United Kingdom) kits, that detect human norovirus (HuNV) antigens in stool samples were evaluated to assess whether they could be used instead of reverse transcription-PCR (RT-PCR) for routine diagnosis. The sensitivities and specificities of the two kits were tested with a panel of 103 stool samples containing HuNVs of 4 and 10 genetic subgroups within genogroups I and II (GI and GII), respectively, and 39 stool samples containing other enteric viruses. The Denka kit had a high sensitivity (>70% for 10 of the 14 subgroups) but a specificity of only 69%, and the Dako kit had a low sensitivity (<30% for 6 GII subgroups) but a high specificity of 100%. Statistical analysis suggests that HuNVs of four subgroups (subgroups GII/2, GII/5, GII/6, and GII/n) are likely to elude detection by the Dako kit. The two kits also demonstrated differences in reactivities. While the Dako kit discriminated between the GI and GII antigens of HuNVs, the Denka kit cross-reacted with samples containing all GI and GII subgroups of HuNVs. Moreover, the Denka kit also reacted with samples containing human sapovirus (HuSV). We demonstrate that the cross-reactivity of the Denka kit is not due to specific reactions with HuNV and HuSV antigens. These results indicate that neither the Denka kit nor the Dako kit has all the performance characteristics required to replace the RT-PCR methods used to detect HuNVs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.