ArticleJournal of clinical microbiology2004
Multiplex PCR for distinguishing the most common phase-1 flagellar antigens of Salmonella spp.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Genetic diversity with potential ESBL-producing and multidrug-resistantFrontiers in veterinary science · 2025Article
- A novel approach for detectingFrontiers in microbiology · 2024Article
- Genotypic virulence profiles and associations in Salmonella isolated from meat samples in wet markets and abattoirs of Metro Manila, Philippines.BMC microbiology · 2022Article
- Antimicrobial Susceptibility and Frequency ofAntibiotics (Basel, Switzerland) · 2021Article
- Article
- Virulence and Antimicrobial Resistance Profiles ofMicroorganisms · 2021Article
- Molecular identification and antibiotic resistance profiling of Salmonella species isolated from chickens in eastern Turkey.BMC veterinary research · 2020Article
- PCR-based detection and serovar identification of Salmonella in retail meat collected from wet markets in Metro Manila, Philippines.PloS one · 2020Article
- Prevalence and molecular characterization of Salmonella enterica serovar Typhimurium from ice and beverages in Jakarta, Indonesia.BMC research notes · 2019Article
- Salmonella Typhimurium in Iran: Contribution of molecular and IS200 PCR methods in variants detection.PloS one · 2019Article
- Identification and phase inversion of Salmonella flagellar antigens, using immuno-discs.The Journal of veterinary medical science · 2018Article
- A simplified multiplex PCR-based typing method for commonThe Indian journal of medical research · 2017Article
- Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods.PloS one · 2017Article
- Article
- Molecular Surveillance Identifies Multiple Transmissions of Typhoid in West Africa.PLoS neglected tropical diseases · 2016Article
- Salmonella Bacteremia Among Children in Central and Northwest Nigeria, 2008-2015.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015Article
- Sequence-level and dual-phase identification of Salmonella flagellum antigens by liquid chromatography-tandem mass spectrometry (LC-MS/MS).Journal of clinical microbiology · 2014Article
- Repression of flagella is a common trait in field isolates of Salmonella enterica serovar Dublin and is associated with invasive human infections.Infection and immunity · 2014Article
- Bovine salmonellosis in northeast of Iran: frequency, genetic fingerprinting and antimicrobial resistance patterns of Salmonella spp.Asian Pacific journal of tropical biomedicine · 2014Article
- Simultaneous and Rapid Detection of Salmonella typhi, Bacillus anthracis, and Yersinia pestis by Using Multiplex Polymerase Chain Reaction (PCR).Iranian Red Crescent medical journal · 2013Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most Salmonella serotypes alternatively express either phase-1 or phase-2 flagellar antigens, encoded by the fliC and fljB genes, respectively. Flagellar phase reversal for the identification of both flagellar antigens is not necessary at the genetic level. Variable internal regions of the fliC genes encoding the H:i, H:r, H:l,v, H:e,h, H:z(10), H:b, and H:d antigens have been sequenced; and the specific sites for each antigen in selected Salmonella serotypes have been determined. These results, together with flagellar G-complex variable internal sequences obtained by the Foodborne and Diarrheal Diseases Branch at the Centers for Disease Control and Prevention in Atlanta, GA, have been used to design a multiplex PCR to identify the G-complex antigens as well as the H:i, H:r, H:l,v, H:e,h, Hz(10), H:b, and H:d first-phase antigens. These antigens are part of the most common Salmonella serotypes possessing first-phase flagellar antigens. Salmonella enterica serotype Enteritidis is identified by adding a specific primer pair published previously. This multiplex PCR includes 13 primers. A total of 161 Salmonella strains associated with 72 different serotypes were tested. Each strain generated one first-phase-specific antigen fragment ranging from 100 to 500 bp; Salmonella serotype Enteritidis, however, generated two amplicons of 500 bp that corresponded to the G complex and a 333-bp serotype-specific amplicon, respectively. Twenty-three strains representing 19 serotypes with flagellar genes different from those targeted in this work did not generate any fragments. The method is quick, specific, and reproducible and is independent of the phase expressed by the bacteria when they are tested.
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