ArticleJournal of clinical microbiology2004
Genomic characterization of an endemic Mycobacterium tuberculosis strain: evolutionary and epidemiologic implications.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Value of pyrazinamide for composition of new treatment regimens for multidrug-resistant Mycobacterium tuberculosis in China.BMC infectious diseases · 2020Article
- Dispersal ofFrontiers in microbiology · 2019Article
- Effects of Host, Sample, andFrontiers in genetics · 2019Article
- Pyrazinamide resistance-conferring mutations in pncA and the transmission of multidrug resistant TB in Georgia.BMC infectious diseases · 2017Article
- Article
- Strain diversity, epistasis and the evolution of drug resistance in Mycobacterium tuberculosis.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2011Review
- Dispersal of Mycobacterium tuberculosis via the Canadian fur trade.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Mycobacterium tuberculosis in Ontario, Canada: Insights from IS6110 restriction fragment length polymorphism and mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping.Journal of clinical microbiology · 2009Article
- Major Mycobacterium tuberculosis lineages associate with patient country of origin.Journal of clinical microbiology · 2009Article
- Does the lipR gene of tubercle bacilli have a role in tuberculosis transmission and pathogenesis?Tuberculosis (Edinburgh, Scotland) · 2009Article
- Progression toward an improved DNA amplification-based typing technique in the study of Mycobacterium tuberculosis epidemiology.Journal of clinical microbiology · 2006Article
- Revisiting the evolution of Mycobacterium bovis.Journal of bacteriology · 2005Article
- Sensitivities and specificities of spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat typing methods for studying molecular epidemiology of tuberculosis.Journal of clinical microbiology · 2005Article
- Article
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7 authors.
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Abstract
In a study of 302 Mycobacterium tuberculosis clinical isolates from the low-incidence Canadian-born population of Quebec, we characterized a large endemic strain family by using genomic deletions. The DS6(Quebec) deleted region (11.4 kb) defined a strain family of 143 isolates encompassing two subgroups: one characterized by pyrazinamide (PZA) susceptibility and the other marked by a PZA-monoresistant phenotype. A second deletion (8 bp) in the pncA gene was shared by all 76 isolates with the PZA resistance phenotype, whereas a third DRv0961 deletion (970 bp) defined a further subset of 15 isolates. From their deletion profiles, we derived a most parsimonious evolutionary scenario and compared multiple standard genotyping modalities (using IS6110 restriction fragment length polymorphism [RFLP], spoligotyping, and mycobacterial interspersed repetitive units [MIRU]) across the deletion-based subgroups. The use of a single genotyping modality yielded an unexpectedly high proportion of clustered isolates for a high IS6110 copy strain (27% by IS6110 RFLP, 61% by MIRU, and 77% by spoligotyping). By combining all three modalities, only 14% were genotypically clustered overall, a result more congruent with the epidemiologic profile of reactivation tuberculosis, as suggested by the older age (mean age, 60 years), rural setting, and low proportion of epidemiologic links. These results provide insight into the evolution of genotypes in endemic strains and the potential for false clustering in molecular epidemiologic studies.
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