ArticleJournal of clinical microbiology2004
Genotypic variation and stability of four variable-number tandem repeats and their suitability for discriminating strains of Mycobacterium leprae.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
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- Article
- Multiple strain infection of Mycobacterium leprae in a family having 4 patients: A study employing short tandem repeats.PloS one · 2019Article
- Genotyping comparison of Mycobacterium leprae isolates by VNTR analysis from nasal samples in a Brazilian endemic region.Pathogens and global health · 2018Article
- Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil.PLoS neglected tropical diseases · 2017Article
- Quantitative lateral flow strip assays as User-Friendly Tools To Detect Biomarker Profiles For Leprosy.Scientific reports · 2016Article
- Sequence Analysis of lip R: A Good Method for Molecular Epidemiology of Clinical Isolates of Mycobacterium tuberculosis.Current microbiology · 2015Article
- Case of diffuse lepromatous leprosy associated with "Mycobacterium lepromatosis".Journal of clinical microbiology · 2011Article
- Genotyping of Mycobacterium leprae from Brazilian leprosy patients suggests the occurrence of reinfection or of bacterial population shift during disease relapse.Journal of medical microbiology · 2011Article
- Molecular typing of Mycobacterium leprae strains from northern India using short tandem repeats.The Indian journal of medical research · 2011Article
- Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay.Journal of clinical microbiology · 2011Article
- Mycobacterium leprae: genes, pseudogenes and genetic diversity.Future microbiology · 2011Review
- Comparative genomic and phylogeographic analysis of Mycobacterium leprae.Nature genetics · 2009Article
- Article
- Population-based molecular epidemiology of leprosy in Cebu, Philippines.Journal of clinical microbiology · 2009Article
- Molecular determination of Mycobacterium leprae viability by use of real-time PCR.Journal of clinical microbiology · 2009Article
- Rapid variable-number tandem-repeat genotyping for Mycobacterium leprae clinical specimens.Journal of clinical microbiology · 2009Article
- Are variable-number tandem repeats appropriate for genotyping Mycobacterium leprae?Journal of clinical microbiology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
It has not been possible to distinguish different strains of Mycobacterium leprae according to their genetic sequence. However, the genome contains several variable-number tandem repeats (VNTR), which have been used effectively in strain typing of other bacteria. To determine their suitability for differentiating M. leprae, we developed PCR systems to amplify 5 different VNTR loci and examined a battery of 12 M. leprae strains derived from patients in different regions of the United States, Brazil, Mexico, and the Philippines, as well as from wild armadillos and a sooty mangabey monkey. We found diversity at four VNTR (D = 0.74), but one system (C(16)G(8)) failed to yield reproducible results. Alleles for the GAA VNTR varied in length from 10 to 16 copies, those for AT(17) varied in length from 10 to 15 copies, those for GTA varied in length from 9 to 12 copies, and those for TA(18) varied in length from 13 to 20 copies. Relatively little variation was seen with interspecies transfer of bacilli or during short-term passage of strains in nude mice or armadillos. The TA(18) locus was more polymorphic than other VNTR, and genotypic variation was more common after long-term expansion in armadillos. Most strain genotypes remained fairly stable in passage, but strain Thai-53 showed remarkable variability. Statistical cluster analysis segregated strains and passage samples appropriately but did not reveal any particular genotype associable with different regions or hosts of origin. VNTR polymorphisms can be used effectively to discriminate M. leprae strains. Inclusion of additional loci and other elements will likely lead to a robust typing system that can be used in community-based epidemiological studies and select clinical applications.
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