ArticleJournal of clinical microbiology2004
Multilocus sequence typing is a reliable alternative method to DNA fingerprinting for discriminating among strains of Candida albicans.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 79 citations in OpenAlex.
- Specific substitutions in the echinocandin target Fks1p account for reduced susceptibility of rare laboratory and clinical Candida sp. isolates.Antimicrobial agents and chemotherapy · 2005Trial
- Antifungal Susceptibility and Multilocus Sequence Typing of Pichia kudriavzevii (Candida krusei) from a Tertiary Hospital in Shanghai.Mycopathologia · 2026Article
- Genotyping ofCurrent medical mycology · 2022Article
- Whole-genome sequence analysis through online web interfaces: a review.Genomics & informatics · 2022Article
- Multilocus sequence typing of Candida albicans isolates from the oral cavities of patients undergoing haemodialysis.Scientific reports · 2018Article
- Phylogenetics of Mycoplasma hominis clinical strains associated with gynecological infections or infertility as disclosed by an expanded multilocus sequence typing scheme.Scientific reports · 2018Article
- Genetic diversity of medically important and emerging Candida species causing invasive infection.BMC infectious diseases · 2015Article
- Optimized multilocus sequence typing (MLST) scheme for Trypanosoma cruzi.PLoS neglected tropical diseases · 2014Article
- Review
- Genetic characterization of Trichomonas vaginalis isolates by use of multilocus sequence typing.Journal of clinical microbiology · 2012Article
- Particular Candida albicans strains in the digestive tract of dyspeptic patients, identified by multilocus sequence typing.PloS one · 2012Article
- Fitness and virulence costs of Candida albicans FKS1 hot spot mutations associated with echinocandin resistance.The Journal of infectious diseases · 2011Article
- Improved detection of Candida sp. fks hot spot mutants by using the method of the CLSI M27-A3 document with the addition of bovine serum albumin.Antimicrobial agents and chemotherapy · 2011Article
- Candida albicans: genotyping methods and clade related phenotypic characteristics.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
- PCR melting profile (PCR MP)--a new tool for differentiation of Candida albicans strains.BMC infectious diseases · 2009Article
- Mating is rare within as well as between clades of the human pathogen Candida albicans.Fungal genetics and biology : FG & B · 2008Article
- Multilocus sequence typing reveals that the population structure of Candida dubliniensis is significantly less divergent than that of Candida albicans.Journal of clinical microbiology · 2008Article
- Analysis of ALS5 and ALS6 allelic variability in a geographically diverse collection of Candida albicans isolates.Fungal genetics and biology : FG & B · 2007Article
- Comparison of microsatellite length polymorphism and multilocus sequence typing for DNA-Based typing of Candida albicans.Journal of clinical microbiology · 2007Article
- MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes.Journal of clinical microbiology · 2007Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multilocus sequence typing (MLST) has emerged as a powerful new DNA-typing tool for the evaluation of intraspecies genetic relatedness. This method relies on DNA sequence analysis of nucleotide polymorphisms in housekeeping genes and has shown a high degree of intraspecies discriminatory power for bacterial and fungal pathogens. However, the results of the MLST scheme for Candida albicans have heretofore never been formally compared to those of other established typing techniques. To assess the value of MLST relative to those of other DNA fingerprinting tools for discriminating among strains of C. albicans, we applied it to a previously well-characterized set of 29 C. albicans isolates evaluated by the random amplified polymorphic DNA (RAPD), multilocus enzyme electrophoresis (MLEE), and Ca3 Southern hybridization probe techniques. MLST identified three clusters of genetically related isolates, with 82.3% direct concordance with MLEE, 82.7% with RAPD analysis, and 86.2% with the Ca3 Southern hybridization technique. When MLST was applied to a subset of 22 isolates of unrelated origins, it identified 21 independent diploid sequence types (DSTs), resulting in a discriminatory power of 99.6%. These DSTs were 96.9, 99.6, and 99.6% concordant with the genotypes identified by RAPD analysis, MLEE, and Ca3 Southern hybridization, respectively. These results demonstrate that MLST is a highly effective technique that performs at least comparably to other established DNA fingerprinting techniques.
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