ArticleJournal of clinical microbiology2004
Molecular characterization of rifampin- and isoniazid-resistant Mycobacterium tuberculosis strains isolated in Poland.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Investigation of Gene Regions Responsible for Drug Resistance in Clinical Isolates ofPathogens (Basel, Switzerland) · 2026Article
- Whole-genome sequencing ofEpidemiology and infection · 2022Article
- Revisiting the methods for detectingAccess microbiology · 2021Review
- Seasonal dynamics in taxonomy and function within bacterial and viral metagenomic assemblages recovered from a freshwater agricultural pond.Environmental microbiome · 2020Article
- A Glutamine Insertion at Codon 432 of RpoB Confers Rifampicin Resistance inFrontiers in microbiology · 2020Article
- Rifamycin congeners kanglemycins are active against rifampicin-resistant bacteria via a distinct mechanism.Nature communications · 2018Article
- Characterization of Mutations Conferring Resistance to Rifampin in Mycobacterium tuberculosis Clinical Strains.Antimicrobial agents and chemotherapy · 2018Article
- Determining Genotypic Drug Resistance by Ion Semiconductor Sequencing With the Ion AmpliSeq™ TB Panel in Multidrug-Resistant Mycobacterium tuberculosis Isolates.Annals of laboratory medicine · 2018Article
- Validation of the FluoroType MTBDR Assay for Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Complex Isolates.Journal of clinical microbiology · 2018Article
- Mutations of rpoB, katG, inhA and ahp genes in rifampicin and isoniazid-resistant Mycobacterium tuberculosis in Kyrgyz Republic.BMC microbiology · 2018Article
- Structural Implications of Mutations Conferring Rifampin Resistance in Mycobacterium leprae.Scientific reports · 2018Article
- Molecular detection of multidrug-resistant tuberculosis among smear-positive pulmonary tuberculosis patients in Jigjiga town, Ethiopia.Infection and drug resistance · 2017Article
- First isolation of Mycobacterium spp. in Mullus spp. in Turkey.Iranian journal of veterinary research · 2015Article
- Sequence analysis for detection of drug resistance in Mycobacterium tuberculosis complex isolates from the Central Region of Cameroon.BMC microbiology · 2014Article
- Occurrence of rpoB mutations in isoniazid-resistant but rifampin-susceptible Mycobacterium tuberculosis isolates from Germany.Antimicrobial agents and chemotherapy · 2014Article
- A systematic follow-up of Mycobacterium tuberculosis drug-resistance and associated genotypic lineages in the French Departments of the Americas over a seventeen-year period.BioMed research international · 2014Article
- The manual mycobacteria growth indicator tube and the nitrate reductase assay for the rapid detection of rifampicin resistance of M. Tuberculosis in low resource settings.BMC infectious diseases · 2012Article
- Visual detection of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis strains by use of an asymmetrically split peroxidase DNAzyme.Journal of clinical microbiology · 2012Article
- Rapid detection of rpoB gene mutations conferring rifampin resistance in Mycobacterium tuberculosis.Journal of clinical microbiology · 2012Article
- Molecular characterization of multidrug-resistant Mycobacterium tuberculosis isolated in Nepal.Antimicrobial agents and chemotherapy · 2012Article
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Authors and funding
8 authors.
Funding
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Abstract
A total of 105 rifampin (RMP)- and/or isoniazid (INH)-resistant strains of Mycobacterium tuberculosis isolated from different parts of Poland in 2000 were screened for mutations associated with resistance to these drugs by two molecular methods, namely sequence analysis and real-time PCR technology. Three loci associated with drug resistance were selected for characterization: they were rpoB (RMP), katG, and the regulatory region of inhA (INH). Nineteen different mutations were identified in 64 RMP-resistant strains, and five new alleles were described. The most common point mutations were in codons 531 (41%), 516 (16%), and 526 (9%) of the rpoB gene. Mutations were not found in two (3%) of the isolates. In the case of resistance to INH, six different mutations in the katG gene of 83 resistant strains were detected. Fifty-seven (69%) isolates exhibited nucleotide substitutions at codon 315. One strain harbored a mutation affecting codon 279 (Gly279Thr). Twelve of 26 INH-resistant strains with the wild-type codon 315 (14.5% of all strains tested) had the mutation -15C-->T in the regulatory region of inhA. A full correlation between the DNA sequence analysis and real-time PCR data was obtained. We conclude that the real-time PCR method is fast and reliable for the detection of RMP and INH resistance-associated mutations in M. tuberculosis clinical isolates.
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