ArticleApplied and environmental microbiology2004
Inactivation of the flagellin gene flaA in Magnetospirillum gryphiswaldense results in nonmagnetotactic mutants lacking flagellar filaments.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 58 citations in OpenAlex.
- Controlling the rotary direction of light-driven molecular motors by topological confinement.Chemical science · 2026Article
- A Two-Protein Chemoreceptor Complex Regulates Oxygen Thresholds in Bacterial Magneto-Aerotaxis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Identification and elimination of genomic regions irrelevant for magnetosome biosynthesis by large-scale deletion in Magnetospirillum gryphiswaldense.BMC microbiology · 2021Article
- Article
- Spatiotemporal Organization of Chemotaxis Pathways in Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2020Article
- Quantifying the Benefit of a Dedicated "Magnetoskeleton" in Bacterial Magnetotaxis by Live-Cell Motility Tracking and Soft Agar Swimming Assay.Applied and environmental microbiology · 2020Article
- High-Throughput Microfluidic Sorting of Live Magnetotactic Bacteria.Applied and environmental microbiology · 2018Article
- Reevaluation of the Complete Genome Sequence of Magnetospirillum gryphiswaldense MSR-1 with Single-Molecule Real-Time Sequencing Data.Genome announcements · 2018Article
- Measurement of the magnetic moment of single Magnetospirillum gryphiswaldense cells by magnetic tweezers.Scientific reports · 2017Article
- Polyhydroxyalkanoate (PHA) Granules Have no Phospholipids.Scientific reports · 2016Article
- Article
- A tailored galK counterselection system for efficient markerless gene deletion and chromosomal tagging in Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2014Article
- Frequent mutations within the genomic magnetosome island of Magnetospirillum gryphiswaldense are mediated by RecA.Journal of bacteriology · 2011Article
- Magnetosome expression of functional camelid antibody fragments (nanobodies) in Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2011Article
- Article
- Cre-lox-based method for generation of large deletions within the genomic magnetosome island of Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2010Article
- Identification of promoters for efficient gene expression in Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2009Article
- Expression of green fluorescent protein fused to magnetosome proteins in microaerophilic magnetotactic bacteria.Applied and environmental microbiology · 2008Article
- The major magnetosome proteins MamGFDC are not essential for magnetite biomineralization in Magnetospirillum gryphiswaldense but regulate the size of magnetosome crystals.Journal of bacteriology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Magnetotactic bacteria synthesize magnetosomes, which cause them to orient and migrate along magnetic field lines. The analysis of magnetotaxis and magnetosome biomineralization at the molecular level has been hindered by the unavailability of genetic methods, namely the lack of a means to introduce directed gene-specific mutations. Here we report a method for knockout mutagenesis by homologous recombination in Magnetospirillum gryphiswaldense. Multiple flagellin genes, which are unlinked in the genome, were identified in M. gryphiswaldense. The targeted disruption of the flagellin gene flaA was shown to eliminate flagella formation, motility, and magnetotaxis. The techniques described in this paper will make it possible to take full advantage of the forthcoming genome sequences of M. gryphiswaldense and other magnetotactic bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.