ArticleApplied and environmental microbiology2004
Novel expression system for large-scale production and purification of recombinant class IIa bacteriocins and its application to piscicolin 126.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Tandem solubility enhancing tags enable the heterologous expression andFrontiers in microbiology · 2026Article
- Improving the Quality and Safety of Fish Products with Edible Coatings Incorporating Piscicolin CM22 from a PsychrotolerantFoods (Basel, Switzerland) · 2024Article
- The Progress of the Biotechnological Production of Class IIa Bacteriocins in Various Cell Factories and Its Future Challenges.International journal of molecular sciences · 2024Review
- Expression and purification of epinecidin-1 variant (Ac-Var-1) by acid cleavage.Applied microbiology and biotechnology · 2024Article
- Understanding the Necessity of Regulatory Protein Machinery in Heterologous Expression of Class-III Type of Ocins.The protein journal · 2023Review
- Antimicrobial Activity and Immunomodulatory Properties of Acidocin A, the Pediocin-like Bacteriocin with the Non-Canonical Structure.Membranes · 2022Article
- High Level Expression and Purification of Cecropin-like Antimicrobial Peptides inBiomedicines · 2022Article
- A novel approach of recombinant laterosporulin production using the N-SH2 domain of SHP-2.BMC biotechnology · 2021Article
- Heterologous Expression of the Class IIa Bacteriocins, Plantaricin 423 and Mundticin ST4SA, inFrontiers in microbiology · 2020Article
- Molecular Characterization and Heterologous Production of the Bacteriocin Peocin, a DNA Starvation/Stationary Phase Protection Protein, fromMolecules (Basel, Switzerland) · 2019Article
- Heterologous expression and purification of BtCspB, a novel cold-shock protein-like bacteriocin from Bacillus thuringiensis BRC-ZYR2.World journal of microbiology & biotechnology · 2019Article
- Purification and Characterization of Plantaricin LPL-1, a Novel Class IIa Bacteriocin Produced byFrontiers in microbiology · 2018Article
- Heterologous Expression of Biopreservative Bacteriocins With a View to Low Cost Production.Frontiers in microbiology · 2018Review
- Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli.Scientific reports · 2017Article
- Rational Design of a Carrier Protein for the Production of Recombinant Toxic Peptides in Escherichia coli.PloS one · 2016Article
- Development of Class IIa Bacteriocins as Therapeutic Agents.International journal of microbiology · 2012Article
- Carrier proteins for fusion expression of antimicrobial peptides in Escherichia coli.Biotechnology and applied biochemistry · 2009Review
- The continuing story of class IIa bacteriocins.Microbiology and molecular biology reviews : MMBR · 2006Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Piscicolin 126 is a class IIa bacteriocin isolated from Carnobacterium piscicola JG126 that exhibits strong activity against Listeria monocytogenes. The gene encoding mature piscicolin 126 (m-pisA) was cloned into an Escherichia coli expression system and expressed as a thioredoxin-piscicolin 126 fusion protein that was purified by affinity chromatography. Purified recombinant piscicolin 126 was obtained after CNBr cleavage of the fusion protein followed by reversed-phase chromatography. Recombinant piscicolin 126 contained a single disulfide bond and had a mass identical to that of native piscicolin 126. This novel bacteriocin expression system generated approximately 26 mg of purified bacteriocin from 1 liter of E. coli culture. The purified recombinant piscicolin 126 acted by disruption of the bacterial cell membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.