ArticleApplied and environmental microbiology2004
Engineering the nifH promoter region and abolishing poly-beta-hydroxybutyrate accumulation in Rhizobium etli enhance nitrogen fixation in symbiosis with Phaseolus vulgaris.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- Engineering Plant-Associated Microorganisms for Bioremediation and Sustainable Agriculture.Microorganisms · 2026Review
- Host-specific fluorescence dynamics in legume-rhizobium symbiosis during nodulation.Applied and environmental microbiology · 2026Article
- Rhizobiome Transplantation: A Novel Strategy beyond Single-Strain/Consortium Inoculation for Crop Improvement.Plants (Basel, Switzerland) · 2023Review
- What determines symbiotic nitrogen fixation efficiency in rhizobium: recent insights into Rhizobium leguminosarum.Archives of microbiology · 2023Review
- How plants recruit their microbiome? New insights into beneficial interactions.Journal of advanced research · 2022Review
- Legume-rhizobium dance: an agricultural tool that could be improved?Microbial biotechnology · 2021Review
- Molecular Biology in the Improvement of Biological Nitrogen Fixation by Rhizobia and Extending the Scope to Cereals.Microorganisms · 2021Review
- Rhamnolipid Enhances the Nitrogen Fixation Activity ofFrontiers in microbiology · 2021Article
- Classical Soybean (International journal of molecular sciences · 2019Article
- Analysis of genome sequence and symbiotic ability of rhizobial strains isolated from seeds of common bean (Phaseolus vulgaris).BMC genomics · 2018Article
- Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases.Journal of molecular evolution · 2015Article
- Nitrogen-fixing rhizobial strains isolated from common bean seeds: phylogeny, physiology, and genome analysis.Applied and environmental microbiology · 2014Article
- PhaP phasins play a principal role in poly-β-hydroxybutyrate accumulation in free-living Bradyrhizobium japonicum.BMC microbiology · 2013Article
- Engineering rhizobial bioinoculants: a strategy to improve iron nutrition.TheScientificWorldJournal · 2013Review
- Polyester synthesis genes associated with stress resistance are involved in an insect-bacterium symbiosis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Computational analysis of common bean (Phaseolus vulgaris L., genotype BAT93) lycopene β-cyclase and β-carotene hydroxylase gene's cDNA.Bioinformation · 2013Article
- Role of trehalose in heat and desiccation tolerance in the soil bacterium Rhizobium etli.BMC microbiology · 2012Article
- Systems biology of bacterial nitrogen fixation: high-throughput technology and its integrative description with constraint-based modeling.BMC systems biology · 2011Article
- Characterization of the NifA-RpoN regulon in Rhizobium etli in free life and in symbiosis with Phaseolus vulgaris.Applied and environmental microbiology · 2010Article
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6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhizobium etli, as well as some other rhizobia, presents nitrogenase reductase (nifH) gene reiterations. Several R. etli strains studied in this laboratory showed a unique organization and contained two complete nifHDK operons (copies a and b) and a truncated nifHD operon (copy c). Expression analysis of lacZ fusion demonstrated that copies a and b in strain CFN42 are transcribed at lower levels than copy c, although this copy has no discernible role during nitrogen fixation. To increase nitrogenase production, we constructed a chimeric nifHDK operon regulated by the strong nifHc promoter sequence and expressed it in symbiosis with the common bean plant (Phaseolus vulgaris), either cloned on a stably inherited plasmid or incorporated into the symbiotic plasmid (pSym). Compared with the wild-type strain, strains with the nitrogenase overexpression construction assayed in greenhouse experiments had, increased nitrogenase activity (58% on average), increased plant weight (32% on average), increased nitrogen content in plants (15% at 32 days postinoculation), and most importantly, higher seed yield (36% on average), higher nitrogen content (25%), and higher nitrogen yield (72% on average) in seeds. Additionally, expression of the chimeric nifHDK operon in a poly-beta-hydroxybutyrate-negative R. etli strain produced an additive effect in enhancing symbiosis. To our knowledge, this is the first report of increased seed yield and nutritional content in the common bean obtained by using only the genetic material already present in Rhizobium.
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