ArticleApplied and environmental microbiology2004
Localization and functional analysis of PepI, the immunity peptide of Pep5-producing Staphylococcus epidermidis strain 5.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
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- GFP fusions of Sec-routed extracellular proteins inMicrobial cell (Graz, Austria) · 2023Article
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- Investigation into the mechanism of action of the antimicrobial peptide epilancin 15X.Frontiers in microbiology · 2023Article
- Current Knowledge of the Mode of Action and Immunity Mechanisms of LAB-Bacteriocins.Microorganisms · 2021Review
- Lipoproteins in Gram-Positive Bacteria: Abundance, Function, Fitness.Frontiers in microbiology · 2020Review
- LanI-Mediated Lantibiotic Immunity in Bacillus subtilis: Functional Analysis.Applied and environmental microbiology · 2019Article
- Antimicrobial Peptide Resistance Mechanisms of Gram-Positive Bacteria.Antibiotics (Basel, Switzerland) · 2014Article
- ApnI, a transmembrane protein responsible for subtilomycin immunity, unveils a novel model for lantibiotic immunity.Applied and environmental microbiology · 2014Article
- Heterologous expression of thuricin CD immunity genes in Listeria monocytogenes.Antimicrobial agents and chemotherapy · 2014Article
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- SmbFT, a putative ABC transporter complex, confers protection against the lantibiotic Smb in Streptococci.Journal of bacteriology · 2013Article
- Cloning and heterologous expression of the thioviridamide biosynthesis gene cluster from Streptomyces olivoviridis.Applied and environmental microbiology · 2013Article
- Characterization of amylolysin, a novel lantibiotic from Bacillus amyloliquefaciens GA1.PloS one · 2013Article
- The First structure of a lantibiotic immunity protein, SpaI from Bacillus subtilis, reveals a novel fold.The Journal of biological chemistry · 2012Article
- Insights into Lantibiotic Immunity Provided by Bioengineering of LtnI.Antimicrobial agents and chemotherapy · 2012Article
- Chemical synthesis and biological activity of analogues of the lantibiotic epilancin 15X.Journal of the American Chemical Society · 2012Article
- Bioengineered nisin A derivatives with enhanced activity against both Gram positive and Gram negative pathogens.PloS one · 2012Article
- Biosynthesis of the antimicrobial peptide epilancin 15X and its N-terminal lactate.Chemistry & biology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pep5 is a cationic pore-forming lantibiotic produced by Staphylococcus epidermidis strain 5. The producer strain protects itself from the lethal action of its own bacteriocin through the 69-amino-acid immunity peptide PepI. The N-terminal segment of PepI contains a 20-amino-acid stretch of apolar residues, whereas the C terminus is very hydrophilic, with a net positive charge. We used green fluorescent protein (GFP)-PepI fusions to obtain information on its localization in vivo. PepI was found to occur outside the cytoplasm and to accumulate at the membrane-cell wall interface. The extracellular localization appeared essential for conferring immunity. We analyzed the functional role of the specific segments by constructing various mutant peptides, which were also fused to GFP. When the hydrophobic N-terminal segment of PepI was disrupted by introducing charged amino acids, the export of PepI was blocked and clones expressing such mutant peptides were Pep5 sensitive. When PepI was successively shortened at the C terminus, in contrast, its export properties remained unchanged whereas its ability to confer immunity was gradually reduced. The results show that the N-terminal part is required for the transport of PepI and that the C-terminal part is important for conferring the immunity phenotype. A concept based on target shielding is proposed for the PepI immunity mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.