ArticleApplied and environmental microbiology2004
Characterization and heterologous gene expression of a novel esterase from Lactobacillus casei CL96.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 68 citations in OpenAlex.
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- Simulation Study for the Degradation of Some Insecticides during Different Black Table Olive Processes.ACS omega · 2020Article
- A novel bacterial carboxylesterase identified in a metagenome derived-clone from Brazilian mangrove sediments.Molecular biology reports · 2020Article
- Characterization of ML-005, a Novel Metaproteomics-Derived Esterase.Frontiers in microbiology · 2018Article
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- Identification of lipolytic enzymes isolated from bacteria indigenous toBiotechnology reports (Amsterdam, Netherlands) · 2017Article
- Antimicrobial peptide gramicidin S is accumulated in granules of producer cells for storage of bacterial phosphagens.Scientific reports · 2017Article
- A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts.Frontiers in microbiology · 2015Article
- Enzymes involved in vinyl acetate decomposition by Pseudomonas fluorescens PCM 2123 strain.Folia microbiologica · 2014Article
- Isolation and characterization of a thermostable esterase from a metagenomic library.Journal of industrial microbiology & biotechnology · 2013Article
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- Identification and characterization of a novel cold-adapted esterase from a metagenomic library of mountain soil.Journal of industrial microbiology & biotechnology · 2012Article
- Comprehensive approaches to molecular biomarker discovery for detection and identification of Cronobacter spp. (Enterobacter sakazakii) and Salmonella spp.Applied and environmental microbiology · 2011Article
- Novel, versatile, and tightly regulated expression system for Escherichia coli strains.Applied and environmental microbiology · 2010Article
- Cloning, expression, purification, and characterization of a novel esterase from Lactobacillus plantarum.Molecular biotechnology · 2010Article
- Heterologous expression of leader-less pga gene in Pichia pastoris: intracellular production of prokaryotic enzyme.BMC biotechnology · 2010Article
- Cloning and characterization of an intracellular esterase from the wine-associated lactic acid bacterium Oenococcus oeni.Applied and environmental microbiology · 2009Article
- Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product.Applied and environmental microbiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel esterase gene (estI) of Lactobacillus casei CL96 was localized on a 3.3-kb BamHI DNA fragment containing an open reading frame (ORF) of 1,800 bp. The ORF of estI was isolated by PCR and expressed in Escherichia coli, the methylotrophic bacterium Methylobacterium extorquens, and the methylotrophic yeast Pichia pastoris under the control of T7, methanol dehydrogenase (P(mxaF)), and alcohol oxidase (AOX1) promoters, respectively. The amino acid sequence of EstI indicated that the esterase is a novel member of the GHSMG family of lipolytic enzymes and that the enzyme contains a lipase-like catalytic triad, consisting of Ser325, Asp516, and His558. E. coli BL21(DE3)/pLysS containing estI expressed a novel 67.5-kDa protein corresponding to EstI in an N-terminal fusion with the S. tag peptide. The recombinant L. casei CL96 EstI protein was purified to electrophoretic homogeneity in a one-step affinity chromatography procedure on S-protein agarose. The optimum pH and temperature of the purified enzyme were 7.0 and 37 degrees C, respectively. Among the pNP (p-nitrophenyl) esters tested, the most selective substrate was pNP-caprylate (C(8)), with K(m) and k(cat) values of 14 +/- 1.08 microM and 1,245 +/- 42.3 S(-1), respectively.
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