ArticleBiological procedures online
Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells.
Article in Biological procedures online. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The trial behind it
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Who cites it
9 citing papers in PubMed.
- Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses.PloS one · 2019Article
- Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice.Archives of virology · 2015Article
- Differential cellular gene expression in duck trachea infected with a highly or low pathogenic H5N1 avian influenza virus.Virology journal · 2013Article
- Differentially expressed genes of human microvascular endothelial cells in response to anti-dengue virus NS1 antibodies by suppression subtractive hybridization.Viral immunology · 2013Article
- In the rat brain acetyl-L-carnitine treatment modulates the expression of genes involved in neuronal ceroid lipofuscinosis.Molecular neurobiology · 2008Article
- Differentially profiling the low-expression transcriptomes of human hepatoma using a novel SSH/microarray approach.BMC genomics · 2006Article
- Generation of subspecies level-specific microbial diagnostic microarrays using genes amplified from subtractive suppression hybridization as microarray probes.Nucleic acids research · 2005Article
- Design and calibration of microarrays as universal transcriptomic environmental biosensors.Comparative and functional genomics · 2005Article
- Discovery of the genes in response to white spot syndrome virus (WSSV) infection in Fenneropenaeus chinensis through cDNA microarray.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High throughput detection of differential expression of genes is an efficient means of identifying genes and pathways that may play a role in biological systems under certain experimental conditions. There exist a variety of approaches that could be used to identify groups of genes that change in expression in response to a particular stimulus or environment. We here describe the application of suppression subtractive hybridization (SSH) coupled with cDNA microarray analysis for isolation and identification of chicken transcripts that change in expression on infection of host cells with a paramyxovirus. SSH was used for initial isolation of differentially expressed transcripts, a large-scale validation of which was accomplished by microarray analysis. The data reveals a large group of regulated genes constituting many biochemical pathways that could serve as targets for future investigations to explore their role in paramyxovirus pathogenesis. The detailed methods described herein could be useful and adaptable to any biological system for studying changes in gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.