ArticlePlanta2004
The MsPRP2 promoter enables strong heterologous gene expression in a root-specific manner and is enhanced by overexpression of Alfin 1.
Article in Planta, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- Analysis of expression characteristics of oil palm EgPRP1 promoter as potential tools for root-trait modification.Plant cell reports · 2026Article
- Genome-Wide Identification of the Alfin-like Gene Family in Cotton (Plants (Basel, Switzerland) · 2024Article
- Genome-wide identification of the plant homeodomain-finger family in rye and ScPHD5 functions in cold tolerance and flowering time.Plant cell reports · 2024Article
- Involvement of Alfin-Like Transcription Factors in Plant Development and Stress Response.Genes · 2024Review
- Expression of Mn-sod, PAL1, aos1 and HPL genes in soybean plants overexpressing the NmDef02 defensin.Transgenic research · 2023Article
- PHD finger proteins function in plant development and abiotic stress responses: an overview.Frontiers in plant science · 2023Review
- Carrot DcALFIN4 and DcALFIN7 Transcription Factors Boost Carotenoid Levels and Participate Differentially in Salt Stress Tolerance When Expressed inInternational journal of molecular sciences · 2022Article
- Assessment of root-specific promoters in banana and tobacco and identification of a banana TIP2 promoter with strong root activity.Frontiers in plant science · 2022Article
- Analysis of expression characteristics of soybean leaf and root tissue-specific promoters in Arabidopsis and soybean.Transgenic research · 2021Article
- Ectopic expression of Medicago truncatula homeodomain finger protein, MtPHD6, enhances drought tolerance in Arabidopsis.BMC genomics · 2019Article
- Identification and characterization ofPeerJ · 2019Article
- An Alfin-like gene from Atriplex hortensis enhances salt and drought tolerance and abscisic acid response in transgenic Arabidopsis.Scientific reports · 2018Article
- GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots.BMC plant biology · 2014Article
- Article
- Very bright orange fluorescent plants: endoplasmic reticulum targeting of orange fluorescent proteins as visual reporters in transgenic plants.BMC biotechnology · 2012Article
- Root-targeted biotechnology to mediate hormonal signalling and improve crop stress tolerance.Plant cell reports · 2011Review
- Soybean GmPHD-type transcription regulators improve stress tolerance in transgenic Arabidopsis plants.PloS one · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Promoter specificity and efficiency of utilization are essential for endogenous and transgene expression. Selective root expression remains to be defined in terms of both promoter elements and transcription factors that provide high levels of ubiquitous expression. We characterized expression from the MsPRP2 promoter with the green fluorescent protein (GFP) reporter transgene in alfalfa (Medicago sativa) and found that a promoter fragment (+1 to -652 bp) retained the root and callus specificity of the endogenous MsPRP2 gene and hence this promoter fragment contains elements necessary for root-specific expression. The strong ubiquitous expression obtained from this promoter was comparable to that of the CaMV 35S promoter in roots and was enhanced by transgenic overexpression of Alfin 1, a root- and callus-specific transcription factor in alfalfa. No transgenic expression was obtained in leaves with this promoter in the presence or absence of Alfin 1. The increased expression of GFP in alfalfa containing the Alfin 1 transgene confirms the function of Alfin 1 binding sites in the MsPRP2 promoter fragment and also indicates that Alfin 1 concentrations are limiting for maximal expression in calli and roots. These findings characterize the MsPRP2 promoter as a novel root- and callus-specific promoter of plant origin that can be used as an effective tool for strong root-directed gene expression. In addition, we have demonstrated that the signal sequence of MsPRP2 can be used for efficient secretion of transgene products from callus and roots.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.