ArticleProceedings of the National Academy of Sciences of the United States of America2004
The topoisomerase IV ParC subunit colocalizes with the Caulobacter replisome and is required for polar localization of replication origins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Review
- Mutational Diversity in the Quinolone Resistance-Determining Regions of Type-II Topoisomerases ofAntibiotics (Basel, Switzerland) · 2021Review
- Replication fork passage drives asymmetric dynamics of a critical nucleoid-associated protein in Caulobacter.The EMBO journal · 2017Article
- A cell cycle-controlled redox switch regulates the topoisomerase IV activity.Genes & development · 2015Article
- Intracellular locations of replication proteins and the origin of replication during chromosome duplication in the slowly growing human pathogen Helicobacter pylori.Journal of bacteriology · 2014Article
- Article
- Structural basis for the MukB-topoisomerase IV interaction and its functional implications in vivo.The EMBO journal · 2013Article
- Sister chromatid interactions in bacteria revealed by a site-specific recombination assay.The EMBO journal · 2012Article
- Getting in the loop: regulation of development in Caulobacter crescentus.Microbiology and molecular biology reviews : MMBR · 2010Review
- Synchronization of chromosome dynamics and cell division in bacteria.Cold Spring Harbor perspectives in biology · 2010Review
- Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli.Molecular cell · 2009Article
- Complex regulatory pathways coordinate cell-cycle progression and development in Caulobacter crescentus.Advances in microbial physiology · 2009Review
- Localization of the naturally occurring plasmid ColE1 at the cell pole.Journal of bacteriology · 2007Article
- GroES/GroEL and DnaK/DnaJ have distinct roles in stress responses and during cell cycle progression in Caulobacter crescentus.Journal of bacteriology · 2006Article
- The bifunctional FtsK protein mediates chromosome partitioning and cell division in Caulobacter.Journal of bacteriology · 2006Article
- Spatial location and requirements for the assembly of the Agrobacterium tumefaciens type IV secretion apparatus.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Novel Ser79Leu and Ser81Ile substitutions in the quinolone resistance-determining regions of ParC topoisomerase IV and GyrA DNA gyrase subunits from recent fluoroquinolone-resistant Streptococcus pneumoniae clinical isolates.Antimicrobial agents and chemotherapy · 2005Article
- Rapid and sequential movement of individual chromosomal loci to specific subcellular locations during bacterial DNA replication.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Linear ordering and dynamic segregation of the bacterial chromosome.Proceedings of the National Academy of Sciences of the United States of America · 2004Review
- Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The process of bacterial DNA replication generates chromosomal topological constraints that are further confounded by simultaneous transcription. Topoisomerases play a key role in ensuring orderly replication and partition of DNA in the face of a continuously changing DNA tertiary structure. In addition to topological constraints, the cellular position of the replication origin is strictly controlled during the cell cycle. In Caulobacter crescentus, the origin of DNA replication is located at the cell pole. Upon initiation of DNA replication, one copy of the duplicated origin sequence rapidly appears at the opposite cell pole. To determine whether the maintenance of DNA topology contributes to the dynamic positioning of a specific DNA region within the cell, we examined origin localization in cells that express temperature-sensitive forms of either the ParC or ParE subunit of topoisomerase (Topo) IV. We found that in the absence of active Topo IV, replication initiation can occur but a significant percent of replication origins are either no longer moved to or maintained at the cell poles. During the replication process, the ParC subunit colocalizes with the replisome, whereas the ParE subunit is dispersed throughout the cell. However, an active ParE subunit is required for ParC localization to the replisome as it moves from the cell pole to the division plane during chromosome replication. We propose that the maintenance of DNA topology throughout the cell cycle contributes to the dynamic positioning of the origin sequence within the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.