ArticleJournal of industrial microbiology & biotechnology2004
A wide-range integrative yeast expression vector system based on Arxula adeninivorans-derived elements.
Article in Journal of industrial microbiology & biotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Engineering the methylotrophic yeastFrontiers in bioengineering and biotechnology · 2023Article
- A New Expression System Based on PsychrotolerantInternational journal of molecular sciences · 2022Article
- Development of a Vector Set for High or Inducible Gene Expression and Protein Secretion in the Yeast GenusJournal of fungi (Basel, Switzerland) · 2022Article
- Yeast Protein as an Easily Accessible Food Source.Metabolites · 2022Review
- Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production.Applied microbiology and biotechnology · 2021Review
- Heterologous coexpression of the benzoate-para-hydroxylase CYP53B1 with different cytochrome P450 reductases in various yeasts.Microbial biotechnology · 2019Article
- Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid.FEMS yeast research · 2018Article
- Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts.Biotechnology for biofuels · 2018Article
- New kids on the block: emerging oleaginous yeast of biotechnological importance.AIMS microbiology · 2017Review
- CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.FEMS yeast research · 2015Article
- Production of a thermostable alcohol dehydrogenase from Rhodococcus ruber in three different yeast species using the Xplor®2 transformation/expression platform.Journal of industrial microbiology & biotechnology · 2012Article
- Application of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts.Microbial cell factories · 2006Article
- Engineering of promoter replacement cassettes for fine-tuning of gene expression in Saccharomyces cerevisiae.Applied and environmental microbiology · 2006Article
- Current awareness on yeast.Yeast (Chichester, England) · 2005Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An Arxula adeninivorans integration vector was applied to a range of alternative yeast species including Saccharomyces cerevisiae, Debaryomyces hansenii, Debaryomyces polymorphus, Hansenula polymorpha and Pichia pastoris. The vector harbours a conserved A. adeninivorans-derived 25S rDNA sequence for targeting, the A. adeninivorans-derived TEF1 promoter for expression control of the reporter sequence, and the Escherichia coli-derived hph gene conferring resistance against hygromycin B for selection of recombinants. Heterologous gene expression was assessed using a green fluorescent protein (GFP) reporter gene. The plasmid was found to be integrated into the genome of the various hosts tested; recombinant strains of all species exhibited heterologous gene expressions of a similar high level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.