ArticleGenes and immunity2004
Expression levels for many genes in human peripheral blood cells are highly sensitive to ex vivo incubation.
Article in Genes and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 170 citations in OpenAlex.
- Peripheral Blood Gene Expression Profiling and Prognostic Significance for the Course of Interstitial Lung Disease in Patients With Systemic Sclerosis.Arthritis & rheumatology (Hoboken, N.J.) · 2025Trial
- Prediction of in vivo radiation dose status in radiotherapy patients using ex vivo and in vivo gene expression signatures.Radiation research · 2011Trial
- Limited dynamic range of immune response gene expression observed in healthy blood donors using RT-PCR.Molecular medicine (Cambridge, Mass.)Trial
- Impact of Low-Dose CT Radiation on Gene Expression and DNA Integrity.International journal of molecular sciences · 2025Article
- Genome-wide transcriptomic response of whole blood to radiation.Scientific reports · 2025Article
- Profiling cell identity and tissue architecture with single-cell and spatial transcriptomics.Nature reviews. Molecular cell biology · 2025Review
- Transcriptomic analysis unveils bona fide molecular signatures of microglia under conditions of homeostasis and viral encephalitis.Journal of neuroinflammation · 2024Article
- FixNCut: single-cell genomics through reversible tissue fixation and dissociation.Genome biology · 2024Article
- Sources of variability in the human platelet transcriptome.Thrombosis research · 2023Review
- Proteome alterations during clonal isolation of established human pancreatic cancer cell lines.Cellular and molecular life sciences : CMLS · 2022Article
- Circular RNAs in peripheral blood mononuclear cells are more stable than linear RNAs upon sample processing delay.Journal of cellular and molecular medicine · 2022Article
- Timing of Blood Sample Processing Affects the Transcriptomic and Epigenomic Profiles in CD4Cells · 2022Article
- The impact of pre-freezing storage time and temperature on gene expression of blood collected in EDTA tubes.Molecular biology reports · 2022Article
- Peripheral blood gene expression profiling shows predictive significance for response to mycophenolate in systemic sclerosis-related interstitial lung disease.Annals of the rheumatic diseases · 2022Article
- Article
- Examination of the differences between sulforaphane and sulforaphene in colon cancer: A study based on next-generation sequencing.Oncology letters · 2021Article
- Assessment of chicken peripheral blood mononuclear cells isolated from freshly drawn blood versus 24 h refrigerated blood.Veterinary world · 2021Article
- Multimodal analysis for humaniScience · 2021Article
- Impact of storage conditions on peripheral leukocytes transcriptome.Molecular biology reports · 2021Article
- Sampling time-dependent artifacts in single-cell genomics studies.Genome biology · 2020Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monitoring of gene and protein expression in peripheral blood cells has significant potential for improving the diagnosis and therapy of many human diseases. As genomic-scale microarray and proteomic technologies are applied to peripheral blood, it is important to consider the variables that may affect interpretation of data. Here we report experiments performed to identify genes that are particularly sensitive to ex vivo handling prior to RNA extraction for gene expression microarrays or quantitative real-time RT-PCR assays. We examined Affymetrix gene expression in samples from eight normal individuals where blood was processed for RNA either immediately after blood draw or the next day following overnight incubation. These studies identified hundreds of genes that are sensitive to ex vivo handling of blood, and suggest that this is an important variable to consider when designing and interpreting human PBMC experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.