ArticleJournal of bacteriology2004
Regulation of hypercompetence in Legionella pneumophila.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Major type IV pilin ofmBio · 2026Article
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- Small molecule communication ofMicrobiology and molecular biology reviews : MMBR · 2024Review
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- FinO/ProQ-family proteins: an evolutionary perspective.Bioscience reports · 2023Review
- Legionnaires' Disease in China Caused byMicroorganisms · 2023Article
- The molecular basis of FimT-mediated DNA uptake during bacterial natural transformation.Nature communications · 2022Article
- The Tail-Specific Protease Is Important for Legionella pneumophila To Survive Thermal Stress in Water and inside Amoebae.Applied and environmental microbiology · 2021Article
- Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation.Journal of bacteriology · 2021Article
- The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches.Frontiers in molecular biosciences · 2021Review
- Structural insight into the membrane targeting domain of the Legionella deAMPylase SidD.PLoS pathogens · 2020Article
- Article
- The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water.Scientific reports · 2018Article
- Silently transformable: the many ways bacteria conceal their built-in capacity of genetic exchange.Current genetics · 2017Review
- ProQ/FinO-domain proteins: another ubiquitous family of RNA matchmakers?Molecular microbiology · 2017Review
- Molecular mechanism of mRNA repression inThe EMBO journal · 2017Article
- PilY1 PromotesFrontiers in cellular and infection microbiology · 2017Article
- Silencing of natural transformation by an RNA chaperone and a multitarget small RNA.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Informatic analysis revealsSynthetic and systems biotechnology · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Although many bacteria are known to be naturally competent for DNA uptake, this ability varies dramatically between species and even within a single species, some isolates display high levels of competence while others seem to be completely nontransformable. Surprisingly, many nontransformable bacterial strains appear to encode components necessary for DNA uptake. We believe that many such strains are actually competent but that this ability has been overlooked because standard laboratory conditions are inappropriate for competence induction. For example, most strains of the gram-negative bacterium Legionella pneumophila are not competent under normal laboratory conditions of aerobic growth at 37 degrees C. However, it was previously reported that microaerophilic growth at 37 degrees C allows L. pneumophila serogroup 1 strain AA100 to be naturally transformed. Here we report that another L. pneumophila serogroup 1 strain, Lp02, can also be transformed under these conditions. Moreover, Lp02 can be induced to high levels of competence by a second set of conditions, aerobic growth at 30 degrees C. In contrast to Lp02, AA100 is only minimally transformable at 30 degrees C, indicating that Lp02 is hypercompetent under these conditions. To identify potential causes of hypercompetence, we isolated mutants of AA100 that exhibited enhanced DNA uptake. Characterization of these mutants revealed two genes, proQ and comR, that are involved in regulating competence in L. pneumophila. This approach, involving the isolation of hypercompetent mutants, shows great promise as a method for identifying natural transformation in bacterial species previously thought to be nontransformable.
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Registered trials
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