ArticleJournal of bacteriology2004
Cellular stoichiometry of the components of the chemotaxis signaling complex.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 145 papers.
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Who cites it
145 citing papers in PubMed, 273 citations in OpenAlex.
- Growth-dependent sensory bet-hedging enhances collective navigation.bioRxiv : the preprint server for biology · 2026Article
- Aer is a bidirectional redox sensor mediating negative chemotaxis to antibiotic-induced ROS inmBio · 2026Article
- Spontaneous switching in a protein signalling array reveals near-critical cooperativity.Nature physics · 2026Article
- Single-array measurements reveal non-uniform, mosaic-like chemosensory arrays in bacteria.Nature communications · 2025Article
- Flavoproteins as native and genetically encoded spin probes for in cell ESR spectroscopy.Nature communications · 2025Article
- Role of a single MCP in evolutionary adaptation ofApplied and environmental microbiology · 2025Article
- Nongenetic adaptation by collective migration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- CheB localizes to polar receptor arrays during repellent adaptation.Science advances · 2024Article
- Article
- Flagellar dynamics reveal fluctuations and kinetic limit in the Escherichia coli chemotaxis network.Scientific reports · 2023Article
- A nonequilibrium allosteric model for receptor-kinase complexes: The role of energy dissipation in chemotaxis signaling.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Trade-offs between cost and information in cellular prediction.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Cellular Abundance of Chemoreceptors, Chemosensory Signaling Proteins, Sensor Histidine Kinases, and Solute Binding Proteins ofInternational journal of molecular sciences · 2023Article
- The ecological roles of bacterial chemotaxis.Nature reviews. Microbiology · 2022Review
- The Effect of the Second Messenger c-di-GMP on Bacterial Chemotaxis in Escherichia coli.Applied and environmental microbiology · 2022Article
- Hexameric rings of the scaffolding protein CheW enhance response sensitivity and cooperativity inScience signaling · 2022Article
- Non-Genetic Diversity in Chemosensing and Chemotactic Behavior.International journal of molecular sciences · 2021Review
- Article
- The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility.The EMBO journal · 2021Article
- Article
85 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
Funding
Abstract
The chemotactic sensory system of Escherichia coli comprises membrane-embedded chemoreceptors and six soluble chemotaxis (Che) proteins. These components form signaling complexes that mediate sensory excitation and adaptation. Previous determinations of cellular content of individual components provided differing and apparently conflicting values. We used quantitative immunoblotting to perform comprehensive determinations of cellular amounts of all components in two E. coli strains considered wild type for chemotaxis, grown in rich and minimal media. Cellular amounts varied up to 10-fold, but ratios between proteins varied no more than 30%. Thus, cellular stoichiometries were almost constant as amounts varied substantially. Calculations using those cellular stoichiometries and values for in vivo proportions of core components in complexes yielded an in vivo stoichiometry for core complexes of 3.4 receptor dimers and 1.6 CheW monomers for each CheA dimer and 2.4 CheY, 0.5 CheZ dimers, 0.08 CheB, and 0.05 CheR per complex. The values suggest a core unit of a trimer of chemoreceptor dimers, a dimer (or two monomers) of kinase CheA, and two CheW. These components may interact in extended arrays and, thus, stoichiometries could be nonintegral. In any case, cellular stoichiometries indicate that CheY could be bound to all signaling complexes and this binding would recruit essentially the entire cellular complement of unphosphorylated CheY, and also that phosphatase CheZ, methylesterase CheB, and methyltransferase CheR would be present at 1 per 2, per 14, and per 20 core complexes, respectively. These characteristic ratios will be important in quantitative treatments of chemotaxis, both experimental and theoretical.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.