ArticleThe Biochemical journal2004
Purification of Drosophila DNA polymerase zeta by REV1 protein-affinity chromatography.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- REV7 associates with ATRIP and inhibits ATR kinase activity.Nucleic acids research · 2026Article
- A sophisticated mechanism governs Pol ζ activity in response to replication stress.Nature communications · 2024Article
- REV7-p53 interaction inhibits ATM-mediated DNA damage signaling.Cell cycle (Georgetown, Tex.) · 2024Article
- REV7 in Cancer Biology and Management.Cancers · 2023Review
- REV7 directs DNA repair pathway choice.Trends in cell biology · 2021Review
- Rescue from replication stress during mitosis.Cell cycle (Georgetown, Tex.) · 2017Review
- REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ.Nucleic acids research · 2015Article
- Structural basis for inhibition of DNA replication by aphidicolin.Nucleic acids research · 2014Article
- REV7 is required for anaphase-promoting complex-dependent ubiquitination and degradation of translesion DNA polymerase REV1.Cell cycle (Georgetown, Tex.) · 2013Article
- Eukaryotic translesion polymerases and their roles and regulation in DNA damage tolerance.Microbiology and molecular biology reviews : MMBR · 2009Review
- REV1 restrains DNA polymerase zeta to ensure frame fidelity during translesion synthesis of UV photoproducts in vivo.Nucleic acids research · 2008Article
- Two X family DNA polymerases, lambda and mu, in meiotic tissues of the basidiomycete, Coprinus cinereus.Chromosoma · 2007Article
- Biochemical properties of a plastidial DNA polymerase of rice.Plant molecular biology · 2007Article
- Novel role for the C terminus of Saccharomyces cerevisiae Rev1 in mediating protein-protein interactions.Molecular and cellular biology · 2006Article
- The critical mutagenic translesion DNA polymerase Rev1 is highly expressed during G(2)/M phase rather than S phase.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Impact of 2-bromo-5,6-dichloro-1-beta-D-ribofuranosyl benzimidazole riboside and inhibitors of DNA, RNA, and protein synthesis on human cytomegalovirus genome maturation.Journal of virology · 2005Article
- Vertebrate DNA damage tolerance requires the C-terminus but not BRCT or transferase domains of REV1.Nucleic acids research · 2005Article
- Review
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies on the biochemical properties of very-large-size eukaryotic DNA polymerases have been limited by the difficulty in obtaining sufficient purified forms of each enzyme. Our aim was to determine and elucidate the biochemical properties of one such polymerase, pol zeta (DNA polymerase zeta) from Drosophila melanogaster (Dmpol zeta). Using an REV1 (UV-revertible gene 1) protein-affinity column, we have isolated the enzyme directly from Drosophila embryos. Completely purified Dmpol zeta was found to have a molecular mass of approx. 240 kDa, and to be sensitive to aphidicolin and resistant to ddTTP (2',3'-dideoxythymidine-5-triphosphate) and N-ethylmaleimide. The enzyme has a preference for poly(dA)/oligo(dT)(10:1) as a template primer and has high processivity for DNA synthesis. Moreover, Dmpol zeta showed significantly higher fidelity compared with Rattus norvegicus DNA polymerase, an error-prone DNA polymerase, in an M13 forward mutation assay. The activities of bypassing pyrimidine dimers and (6-4) photoproducts and extending from mismatched primer-template termini in (6-4) photoproduct by Dmpol zeta were not detected. Drosophila REV7 interacted with Dmpol zeta in vitro, but did not influence the DNA synthesis activity of Dmpol zeta. The present study is the first report about characterization of purified pol zeta from multicellular organisms, and the second concerning the characterization of yeast pol zeta.
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