ArticleThe Journal of cell biology2004
Bld10p, a novel protein essential for basal body assembly in Chlamydomonas: localization to the cartwheel, the first ninefold symmetrical structure appearing during assembly.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
71 citing papers in PubMed, 142 citations in OpenAlex.
- Genome editing in the green alga Chlamydomonas: past, present practice and future prospects.The Plant journal : for cell and molecular biology · 2025Review
- Article
- Review
- Swimming ability and flagellar motility of sperm packets of the volvocine green alga Pleodorina starrii.PloS one · 2024Article
- Towards understanding centriole elimination.Open biology · 2023Review
- Role of intraflagellar transport in transcriptional control during flagellar regeneration inMolecular biology of the cell · 2023Article
- Bld10p/Cep135 determines the number of triplets in the centriole independently of the cartwheel.The EMBO journal · 2022Article
- Ciliate cortical organization and dynamics for cell motility: Comparing ciliates and vertebrates.The Journal of eukaryotic microbiology · 2022Review
- A modified TurboID approach identifies tissue-specific centriolar components in C. elegans.PLoS genetics · 2022Article
- Human centrosome organization and function in interphase and mitosis.Seminars in cell & developmental biology · 2021Review
- Autosomal Recessive Primary Microcephaly: Not Just a Small Brain.Frontiers in cell and developmental biology · 2021Review
- Architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography.The EMBO journal · 2020Article
- WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.eLife · 2020Article
- With Age Comes Maturity: Biochemical and Structural Transformation of a Human Centriole in the Making.Cells · 2020Review
- CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions.Structure (London, England : 1993) · 2020Article
- Regulated protein stabilization underpins the functional interplay among basal body components inThe Journal of biological chemistry · 2020Article
- Article
- CEP135 isoform dysregulation promotes centrosome amplification in breast cancer cells.Molecular biology of the cell · 2019Article
- Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism.eLife · 2019Article
- Motile Cilia: Innovation and Insight From Ciliate Model Organisms.Frontiers in cell and developmental biology · 2019Review
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
How centrioles and basal bodies assemble is a long-standing puzzle in cell biology. To address this problem, we analyzed a novel basal body-defective Chlamydomonas reinhardtii mutant isolated from a collection of flagella-less mutants. This mutant, bld10, displayed disorganized mitotic spindles and cytoplasmic microtubules, resulting in abnormal cell division and slow growth. Electron microscopic observation suggested that bld10 cells totally lack basal bodies. The product of the BLD10 gene (Bld10p) was found to be a novel coiled-coil protein of 170 kD. Immunoelectron microscopy localizes Bld10p to the cartwheel, a structure with ninefold rotational symmetry positioned near the proximal end of the basal bodies. Because the cartwheel forms the base from which the triplet microtubules elongate, we suggest that Bld10p plays an essential role in an early stage of basal body assembly. A viable mutant having such a severe basal body defect emphasizes the usefulness of Chlamydomonas in studying the mechanism of basal body/centriole assembly by using a variety of mutants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.