ArticleGenome research2004
DNA mapping using microfluidic stretching and single-molecule detection of fluorescent site-specific tags.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 162 citations in OpenAlex.
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- Modelling BioNano optical data and simulation study of genome map assembly.Bioinformatics (Oxford, England) · 2018Article
- Optimization of flow-focusing devices for homogeneous extensional flow.Biomicrofluidics · 2018Article
- Analysis of single nucleic acid molecules in micro- and nano-fluidics.Lab on a chip · 2016Review
- Article
- Visualizing the entire DNA from a chromosome in a single frame.Biomicrofluidics · 2015Article
- Locked nucleic acid oligomers as handles for single molecule manipulation.Nucleic acids research · 2014Article
- Toward single-molecule optical mapping of the epigenome.ACS nano · 2014Article
- Shadow masking for nanomaterial-based biosensors incorporated with a microfluidic device.Biomedical microdevices · 2013Article
- Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.Chemical reviews · 2013Review
- The Fluid Mechanics of Genome Mapping.AIChE journal. American Institute of Chemical Engineers · 2013Article
- Stretching DNA by electric field and flow field in microfluidic devices: An experimental validation to the devices designed with computer simulations.Biomicrofluidics · 2013Article
- A dual-mode single-molecule fluorescence assay for the detection of expanded CGG repeats in Fragile X syndrome.Molecular biotechnology · 2013Article
- Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells.Lab on a chip · 2012Article
- Genome mapping on nanochannel arrays for structural variation analysis and sequence assembly.Nature biotechnology · 2012Article
- Multicolor super-resolution DNA imaging for genetic analysis.Nano letters · 2012Article
- Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins.Angewandte Chemie (International ed. in English) · 2012Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a rapid molecular mapping technology--Direct Linear Analysis (DLA)--on the basis of the analysis of individual DNA molecules bound with sequence-specific fluorescent tags. The apparatus includes a microfluidic device for stretching DNA molecules in elongational flow that is coupled to a multicolor detection system capable of single-fluorophore sensitivity. Double-stranded DNA molecules were tagged at sequence-specific motif sites with fluorescent bisPNA (Peptide Nucleic Acid) tags. The DNA molecules were then stretched in the microfluidic device and driven in a flow stream past confocal fluorescence detectors. DLA provided the spatial locations of multiple specific sequence motifs along individual DNA molecules, and thousands of individual molecules could be analyzed per minute. We validated this technology using the 48.5 kb lambda phage genome with different 8-base and 7-base sequence motif tags. The distance between the sequence motifs was determined with an accuracy of +/-0.8 kb, and these tags could be localized on the DNA with an accuracy of +/-2 kb. Thus, DLA is a rapid mapping technology, suitable for analysis of long DNA molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.