ArticleBiochemistry2004
Recognition of anionic phospholipid membranes by an antihemostatic protein from a blood-feeding insect.
Article in Biochemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- A combined transcriptomic approach to identify candidates for an anti-tick vaccine blocking B. afzelii transmission.Scientific reports · 2020Article
- Electrostatic Tuning of the Ligand Binding Mechanism by Glu27 in Nitrophorin 7.Scientific reports · 2018Article
- Thrombin inhibitor from the salivary gland of the camel tick Hyalomma dromedarii.Experimental & applied acarology · 2018Article
- A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease.PLoS neglected tropical diseases · 2016Article
- Article
- Review
- Dimerization of nitrophorin 4 at low pH and comparison to the K1A mutant of nitrophorin 1.Biochemistry · 2015Article
- Structure and dynamics of the membrane attaching nitric oxide transporter nitrophorin 7.F1000Research · 2015Article
- Article
- De novo Ixodes ricinus salivary gland transcriptome analysis using two next-generation sequencing methodologies.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Novel family of insect salivary inhibitors blocks contact pathway activation by binding to polyphosphate, heparin, and dextran sulfate.Arteriosclerosis, thrombosis, and vascular biology · 2013Article
- Crystallization and preliminary X-ray crystallographic analysis of the membrane-binding haemprotein nitrophorin 7 from Rhodnius prolixus.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Alboserpin, a factor Xa inhibitor from the mosquito vector of yellow fever, binds heparin and membrane phospholipids and exhibits antithrombotic activity.The Journal of biological chemistry · 2011Article
- A deep insight into the sialotranscriptome of the gulf coast tick, Amblyomma maculatum.PloS one · 2011Article
- Structure and mechanism in salivary proteins from blood-feeding arthropods.Toxicon : official journal of the International Society on Toxinology · 2010Review
- Platelet aggregation inhibitors from hematophagous animals.Toxicon : official journal of the International Society on Toxinology · 2010Review
- An insight into the sialotranscriptome of Simulium nigrimanum, a black fly associated with fogo selvagem in South America.The American journal of tropical medicine and hygiene · 2010Article
- Ultrafast dynamics of diatomic ligand binding to nitrophorin 4.Journal of the American Chemical Society · 2010Article
- 1H and 13C NMR spectroscopic studies of the ferriheme resonances of three low-spin complexes of wild-type nitrophorin 2 and nitrophorin 2(V24E) as a function of pH.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2009Article
- Protein dynamics of a beta-sheet protein.European biophysics journal : EBJ · 2009Article
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Authors and funding
5 authors.
Funding
Abstract
The saliva of blood-feeding insects contains a variety of molecules having antihemostatic activity. Here, we describe nitrophorin 7 (NP7), a salivary protein that binds with high affinity to anionic phospholipid membranes. The protein is apparently targeted to the negatively charged surfaces of activated platelets and other cells, where it can serve as a vasodilator, antihistamine, platelet aggregation inhibitor, and anticoagulant. As with other members of the nitrophorin group, NP7 reversibly binds a molecule of NO and binds histamine with high affinity. The protein differs from other nitrophorins in that it binds to membranes containing phosphatidylserine. Sedimentation and surface plasmon resonance experiments, revealed two classes of phospholipid-binding sites having K(d) values of 4.8 and 755 nM. NP7 inhibits prothrombin activation by blocking phospholipid binding sites for the prothrombinase complex on the surfaces of vesicles and activated platelets. As a NO complex, NP7 inhibits collagen and ADP-induced platelet aggregation and induces disaggregation of ADP-stimulated platelets by an NO-mediated mechanism. Molecular modeling of NP7 revealed a putative, positively charged membrane interaction surface comprised mainly of a helix lying outside of the lipocalin beta-barrel structure.
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