ArticleMolecular and cellular biology2004
A new Saccharomyces cerevisiae strain with a mutant Smt3-deconjugating Ulp1 protein is affected in DNA replication and requires Srs2 and homologous recombination for its viability.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Comparative Analysis of Lifecycle-Dependent A to I RNA Editing in Three Members of theJournal of fungi (Basel, Switzerland) · 2026Article
- Dynamic sumoylation is essential in yeast for suppressing stress-induced gene expression in non-stress conditions.Nucleic acids research · 2026Article
- A novel lncRNA YIL163C enhances genomic stability and antifungal resistance via the DNA damage response inFrontiers in microbiology · 2025Article
- 1,10-phenanthroline inhibits sumoylation and reveals that yeast SUMO modifications are highly transient.EMBO reports · 2024Article
- Sumoylation is Largely Dispensable for Normal Growth but Facilitates Heat Tolerance in Yeast.Molecular and cellular biology · 2023Article
- Review
- A Lysine Desert Protects a Novel Domain in the Slx5-Slx8 SUMO Targeted Ub Ligase To Maintain Sumoylation Levels inGenetics · 2017Article
- SUMO and the robustness of cancer.Nature reviews. Cancer · 2017Review
- Wrestling with Chromosomes: The Roles of SUMO During Meiosis.Advances in experimental medicine and biology · 2017Review
- Identification of SUMO modification sites in the base excision repair protein, Ntg1.DNA repair · 2016Article
- Functions of Ubiquitin and SUMO in DNA Replication and Replication Stress.Frontiers in genetics · 2016Review
- Interplay between sumoylation and phosphorylation for protection against α-synuclein inclusions.The Journal of biological chemistry · 2014Article
- Evolutionarily conserved genetic interactions with budding and fission yeast MutS identify orthologous relationships in mismatch repair-deficient cancer cells.Genome medicine · 2014Article
- SUMO Wrestles with Recombination.Biomolecules · 2012Article
- Homologous recombination and its regulation.Nucleic acids research · 2012Review
- The SUMO isopeptidase Ulp2p is required to prevent recombination-induced chromosome segregation lethality following DNA replication stress.PLoS genetics · 2011Article
- Nuclear organization in genome stability: SUMO connections.Cell research · 2011Review
- Wss1 is a SUMO-dependent isopeptidase that interacts genetically with the Slx5-Slx8 SUMO-targeted ubiquitin ligase.Molecular and cellular biology · 2010Article
- Genome stability roles of SUMO-targeted ubiquitin ligases.DNA repair · 2009Review
- Functional targeting of DNA damage to a nuclear pore-associated SUMO-dependent ubiquitin ligase.Science (New York, N.Y.) · 2008Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Saccharomyces cerevisiae Srs2 protein is involved in DNA repair and recombination. In order to gain better insight into the roles of Srs2, we performed a screen to identify mutations that are synthetically lethal with an srs2 deletion. One of them is a mutated allele of the ULP1 gene that encodes a protease specifically cleaving Smt3-protein conjugates. This allele, ulp1-I615N, is responsible for an accumulation of Smt3-conjugated proteins. The mutant is unable to grow at 37 degrees C. At permissive temperatures, it still shows severe growth defects together with a strong hyperrecombination phenotype and is impaired in meiosis. Genetic interactions between ulp1 and mutations that affect different repair pathways indicated that the RAD51-dependent homologous recombination mechanism, but not excision resynthesis, translesion synthesis, or nonhomologous end-joining processes, is required for the viability of the mutant. Thus, both Srs2, believed to negatively control homologous recombination, and the process of recombination per se are essential for the viability of the ulp1 mutant. Upon replication, mutant cells accumulate single-stranded DNA interruptions. These structures are believed to generate different recombination intermediates. Some of them are fixed by recombination, and others require Srs2 to be reversed and fixed by an alternate pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.