ArticleThe Journal of physiology2004
Isoflurane depresses glutamate release by reducing neuronal excitability at the Drosophila neuromuscular junction.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 63 citations in OpenAlex.
- Parvalbumin interneuron activity drives fast inhibition-induced vasoconstriction followed by slow substance P-mediated vasodilation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Microfluidics for understanding model organisms.Nature communications · 2022Review
- Opposed hemodynamic responses following increased excitation and parvalbumin-based inhibition.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2021Article
- Integrated information structure collapses with anesthetic loss of conscious arousal in Drosophila melanogaster.PLoS computational biology · 2021Article
- LarvaSPA, A Method for Mounting Drosophila Larva for Long-Term Time-Lapse Imaging.Journal of visualized experiments : JoVE · 2020Article
- On chip cryo-anesthesia of Drosophila larvae for high resolution in vivo imaging applications.Lab on a chip · 2017Article
- Regulation of quantal currents determines synaptic strength at neuromuscular synapses in larval Drosophila.Pflugers Archiv : European journal of physiology · 2016Article
- Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons.Cell reports · 2016Article
- Effects of isoflurane and ethanol administration on c-Fos immunoreactivity in mice.Neuroscience · 2016Article
- Article
- Using microfluidics chips for live imaging and study of injury responses in Drosophila larvae.Journal of visualized experiments : JoVE · 2014Article
- Article
- Kismet positively regulates glutamate receptor localization and synaptic transmission at the Drosophila neuromuscular junction.PloS one · 2014Article
- Drosophila ryanodine receptors mediate general anesthesia by halothane.Anesthesiology · 2013Article
- Drosophila neuroligin 1 regulates synaptic growth and function in response to activity and phosphoinositide-3-kinase.Molecular and cellular neurosciences · 2012Article
- Anesthesia and the quantitative evaluation of neurovascular coupling.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2012Review
- Microfluidic chips for in vivo imaging of cellular responses to neural injury in Drosophila larvae.PloS one · 2012Article
- Extracellular protons reduce quantal content and prolong synaptic currents at the Drosophila larval neuromuscular junction.Journal of neurogenetics · 2011Article
- NFAT regulates pre-synaptic development and activity-dependent plasticity in Drosophila.Molecular and cellular neurosciences · 2011Article
- The RhoGAP crossveinless-c interacts with Dystrophin and is required for synaptic homeostasis at the Drosophila neuromuscular junction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
The mechanisms through which volatile general anaesthetics exert their behavioural effects remain unclear. The accessibility of the Drosophila larval neuromuscular junction to genetic and neurophysiological analysis has made it an attractive model system for identification of anaesthetic targets. This study provides a mechanistic basis for the genetic analysis of anaesthetic action, by analysing the neurophysiological effects of the volatile anaesthetic isoflurane on axonal and synaptic function in the Drosophila larva. The most robust effect of isoflurane was a reversible decrease in the amplitude and area of glutamatergic excitatory junctional currents (EJCs) evoked at the neuromuscular junction. Isoflurane did not affect postsynaptic glutamate receptor function detectably, in that the amplitudes, areas and decay times of spontaneous miniature EJCs were unchanged at any concentration. Therefore, decreased EJC amplitude resulted from reduction of neurotransmitter release. Reduced neurotransmitter release was associated with decreased presynaptic excitability, measured as increased delay to EJC onset and reduced axonal conduction velocity. EJC amplitude was rescued to control levels by direct electrotonic stimulation of the synapse in the presence of tetrodotoxin, indicating that isoflurane inhibits neurotransmitter release by reducing presynaptic excitability. In addition, isoflurane reduced release probability, measured as increased paired-pulse facilitation. The EC(50) for suppression of larval locomotion was similar to that for reduction of transmitter release, indicating that the axonal and synaptic effects were occurring in a behaviourally relevant range. These results provide a cellular context for ongoing genetic and neurophysiological analyses of volatile anaesthetic action in Drosophila, and suggest candidate anaesthetic target molecules.
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