Evidence map›Paper›PMID 15169571›Full record

ArticleRetrovirology2004

A murine leukemia virus with Cre-LoxP excisible coding sequences allowing superinfection, transgene delivery, and generation of host genomic deletions.

Clifford L Wang, J Graeme Hodgson, Tiffany Malek, Finn Skou Pedersen, Matthias Wabl

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Retrovirology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Clifford L WangDepartment of Microbiology and Immunology, University of California, San Francisco 94143-0414, USA. cliffw@itsa.ucsf.edu
J Graeme Hodgson
Tiffany Malek
Finn Skou Pedersen
Matthias Wabl
University of California, San Francisco · USAarhus University · DKFred Hutch Cancer Center · US

Funding

Genetic Variation in B-Lymphocyte OntogenyR01AG020684 · NIA · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI WABL, MATTHIAS R · 2001 to 2005
$1.7M
NIA NIH HHS AG20684NIA NIH HHS R01 AG020684
6 · The paper itself

Abstract

backgroundTo generate a replication-competent retrovirus that could be conditionally inactivated, we flanked the viral genes of the Akv murine leukemia virus with LoxP sites. This provirus can delete its envelope gene by LoxP/Cre mediated recombination and thereby allow superinfection of Cre recombinase expressing cells.

resultsIn our studies, the virus repeatedly infected the cell and delivered multiple copies of the viral genome to the host genome; the superinfected cells expressed a viral transgene on average twenty times more than non-superinfected cells. The insertion of multiple LoxP sites into the cellular genome also led to genomic deletions, as demonstrated by comparative genome hybridization.

conclusionWe envision that this technology may be particularly valuable for delivering transgenes and/or causing deletions.

Indexed as

Gene Deletion3T3 CellsAnimalsGenes, envGenetic VectorsGenome, ViralIntegrasesLeukemia Virus, MurineMiceSequence DeletionSuperinfectionTransfectionViral ProteinsCre recombinaseIntegrasesViral Proteins

Identifiers

PMID15169571
PMCPMC419723
OpenAlexW2148128170

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.