ArticleClinical & experimental metastasis2004
A soybean Kunitz trypsin inhibitor suppresses ovarian cancer cell invasion by blocking urokinase upregulation.
Article in Clinical & experimental metastasis, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 94 citations in OpenAlex.
- A Review of Bioactive Compound Effects from Primary Legume Protein Sources in Human and Animal Health.Current issues in molecular biology · 2024Review
- An Effective Modification Strategy to Build Multifunctional Peptides Based on a Trypsin Inhibitory Peptide of the Kunitz Family.Pharmaceutics · 2024Article
- A Novel Trypsin Kunitz-Type Inhibitor from Cajanus cajan Leaves and Its Inhibitory Activity on New Cancer Serine Proteases and Its Effect on Tumor Cell Growth.The protein journal · 2024Article
- Bikunin: A Promising Prognostic Biomarker for Acute-on-Chronic Liver Failure in Patients with Viral Hepatitis B.Infection and drug resistance · 2023Article
- Proliferation and Invasion of Melanoma Are Suppressed by a Plant Protease Inhibitor, Leading to Downregulation of Survival/Death-Related Proteins.Molecules (Basel, Switzerland) · 2022Article
- The Hydrolytic Peptides of Soybean Protein Induce Cell Cycle Arrest and Apoptosis on Human Oral Cancer Cell Line HSC-3.Molecules (Basel, Switzerland) · 2022Article
- Protease Inhibitors from Plants as Therapeutic Agents- A Review.Plant foods for human nutrition (Dordrecht, Netherlands) · 2022Review
- Unravelling theMolecules (Basel, Switzerland) · 2022Article
- Urease andEvidence-based complementary and alternative medicine : eCAM · 2022Article
- Effect of freezing treatment of soybean on soymilk nutritional components, protein digestibility, and functional components.Food science & nutrition · 2021Article
- Structure-Activity Relationship and Molecular Docking of a Kunitz-Like Trypsin Inhibitor, Kunitzin-AH, from the Skin Secretion ofPharmaceutics · 2021Article
- Preparation and Irreversible Inhibition Mechanism Insight into a Recombinant Kunitz Trypsin Inhibitor from Glycine max L. Seeds.Applied biochemistry and biotechnology · 2020Article
- Cancer Stem Cells: The Potential Targets of Chinese Medicines and Their Active Compounds.International journal of molecular sciences · 2016Review
- Plant Protease Inhibitors in Therapeutics-Focus on Cancer Therapy.Frontiers in pharmacology · 2016Review
- Anticancer effects of sweet potato protein on human colorectal cancer cells.World journal of gastroenterology · 2013Article
- Bioactive peptides and depsipeptides with anticancer potential: sources from marine animals.Marine drugs · 2012Review
- Isolation and characterization of a Kunitz-type trypsin inhibitor with antiproliferative activity from Gymnocladus chinensis (Yunnan bean) seeds.The protein journal · 2011Article
- Antitumor and HIV-1 Reverse Transcriptase Inhibitory Activities of a Hemagglutinin and a Protease Inhibitor from Mini-Black Soybean.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- Trypsin isoinhibitors with antiproliferative activity toward leukemia cells from Phaseolus vulgaris cv "White Cloud Bean".Journal of biomedicine & biotechnology · 2010Article
- Purification of melibiose-binding lectins from two cultivars of Chinese black soybeans.Acta biochimica et biophysica Sinica · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
We have previously reported in a series of papers that a Kunitz-type protease inhibitor, bikunin, suppresses up-regulation of urokinase-type plasminogen activator (uPA) and its specific receptor (uPAR) expression, phosphorylation of ERK1/2 and cancer cell invasion in vitro and peritoneal disseminated metastasis in vivo. In the present study, we investigated the effects of soy bean trypsin inhibitor (SBTI) on the net enzymatic activity of secreted, extracellular uPA, signal transduction involved in the expression of uPA and invasion in HRA human ovarian cancer cells. SBTI contains a Kunitz trypsin inhibitor (KTI) and a Bowman-Birk inhibitor (BBI). Here, we show 1) that KTI and BBI were purified separately from soybeans; 2) that neither KTI nor BBI effectively inhibits enzymatic activity of uPA; 3) that uPA upregulation observed in HRA cells was inhibited by preincubation of the cells with KTI with an IC50 of approximately 2 microM, whereas BBI failed to repress uPA upregulation, as measured by enzyme-linked immunosorbent assay; 4) that cell invasiveness was inhibited by treatment of the cells with KTI with an IC50 of approximately 3 microM, whereas BBI failed to suppress cell invasion, as measured by an in vitro invasion assay; 5) KTI suppresses HRA cell invasion by blocking uPA up-regulation which may be mediated by a binding protein(s) other than a bikunin binding protein and/or its receptor; and 6) that transforming growth factor-beta 1 (TGF-beta1)-mediated activation of ERK1/2 was significantly reduced by preincubation of the cells with KTI. In conclusion, KTI, but not BBI, could inhibit cell invasiveness at least through suppression of uPA signaling cascade, although the mechanisms of KTI may be different from those of bikunin.
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