ArticlePlant cell reports2004
Use of the tobacco feedback-insensitive anthranilate synthase gene (ASA2) as a selectable marker for legume hairy root transformation.
Article in Plant cell reports, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.Journal of biosciences · 2012Review
- High level transgenic expression of soybean (Glycine max) GmERF and Gmubi gene promoters isolated by a novel promoter analysis pipeline.BMC plant biology · 2010Article
- Rapid in planta evaluation of root expressed transgenes in chimeric soybean plants.Plant cell reports · 2010Article
- Lysine racemase: a novel non-antibiotic selectable marker for plant transformation.Plant molecular biology · 2010Article
- Tobacco plastid transformation using the feedback-insensitive anthranilate synthase [alpha]-subunit of tobacco (ASA2) as a new selectable marker.Journal of experimental botany · 2009Article
- Tissue culture specificity of the tobacco ASA2 promoter driving hpt as a selectable marker for soybean transformation selection.Plant cell reports · 2008Article
- Expression of a feedback insensitive anthranilate synthase gene from tobacco increases free tryptophan in soybean plants.Plant cell reports · 2007Article
- Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation.Planta · 2007Article
- Article
- Overexpression of the feedback-insensitive anthranilate synthase gene in tobacco causes tryptophan accumulation.Plant cell reports · 2005Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The feedback-insensitive anthranilate synthase ( ASA2) cDNA--isolated from a 5-methyltryptophan (5MT)-resistant tobacco cell line--driven by the CaMV 35S promoter or 606 bp of the native ASA2 promoter, was introduced into the forage legume plant Astragalus sinicus or soybean ( Glycine max), using Agrobacterium rhizogenes strains DC-AR2 or K599, respectively. Hairy roots of A. sinicus transformed with 35S-ASA2 but not 606- ASA2 could be directly selected using 20-75 micro M 5MT. ASA2 mRNA was expressed in all A. sinicus lines selected with 5MT, but nptII mRNA was expressed only in some lines even though the gene was present. Free tryptophan was increased 8- to 26-fold in A. sinicus and 3- to 6-fold in soybean (selected with kanamycin). An HPLC method was used to measure anthranilate synthase (AS) activity since there was a fluorescent compound or compounds present in the soybean hairy root extracts. The transformed soybean hairy roots contained more feedback-resistant AS activity, showing that there is interaction of the tobacco ASA2 alpha-subunit with the soybean beta-subunit to form an active enzyme. Soybean hairy roots that express ASA2 also exhibit 5MT resistance. These results demonstrate that the tobacco feedback-insensitive ASA2 gene can be used as a selectable marker for transformation of the legume A. sinicus.
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