ArticleDiabetologia1992
Immunocytochemical localization of heat-shock protein 60-related protein in beta-cell secretory granules and its altered distribution in non-obese diabetic mice.
Article in Diabetologia, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 68 citations in OpenAlex.
- Trial
- Proteins Differentially Expressed in the Pancreas of Hepatic Alcohol Dehydrogenase-Deficient Deer Mice Fed Ethanol For 3 Months.Pancreas · 2017Article
- Unconventional Secretion of Heat Shock Proteins in Cancer.International journal of molecular sciences · 2017Review
- Multifactorial comparative proteomic study of cytochrome P450 2E1 function in chronic alcohol administration.PloS one · 2014Article
- The Mycobacterium bovis bacille Calmette-Guerin phagosome proteome.Molecular & cellular proteomics : MCP · 2010Article
- Chlamydia trachomatis infection and anti-Hsp60 immunity: the two sides of the coin.PLoS pathogens · 2009Review
- Insulin Dependant Gene Expression of Heat Shock Protein 60 in H4IIE Hepatoma Cells.International journal of clinical and experimental medicine · 2008Article
- On the brotherhood of the mitochondrial chaperones mortalin and heat shock protein 60.Cell stress & chaperones · 2006Review
- Localization of mitochondrial DNA encoded cytochrome c oxidase subunits I and II in rat pancreatic zymogen granules and pituitary growth hormone granules.Histochemistry and cell biology · 2005Article
- Expression differences in mitochondrial and secretory chaperonin 60 (Cpn60) in pancreatic acinar cells.Cell stress & chaperones · 2003Article
- Plasma membrane expression of heat shock protein 60 in vivo in response to infection.Infection and immunity · 1999Article
- Role of heat shock proteins in protection from and pathogenesis of infectious diseases.Clinical microbiology reviews · 1999Review
- Protein kinase A-catalyzed phosphorylation of heat shock protein 60 chaperone regulates its attachment to histone 2B in the T lymphocyte plasma membrane.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Genetic background and environment contribute synergistically to the onset of autoimmune diseases.Journal of molecular medicine (Berlin, Germany) · 1995Review
- Heat shock induces resistance in rat pancreatic islet cells against nitric oxide, oxygen radicals and streptozotocin toxicity in vitro.The Journal of clinical investigation · 1995Article
- Heat-Shock Stress-Response Proteins in Endocrine Pathology.Endocrine pathology · 1995Article
- Enhancement in amount of P1 (hsp60) in mutants of Chinese hamster ovary (CHO-K1) cells exhibiting increases in the A system of amino acid transport.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Synaptophysin-containing microvesicles transport heat-shock protein hsp60 in insulin-secreting beta cells.Cytotechnology · 1993Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immuno-electron microscopy technique was employed to investigate the cellular distribution of 60 kDa heat-shock protein (HSP60) in pancreatic Beta cells of control and non-obese diabetic mice. In thin sections prepared from control mice, antibody to mammalian HSP60 cross-reacted with protein(s) located to mitochondria and secretory granules. In particular, prominent binding of the antibody was seen to the insulin core of the mature insulin-secreting granules. In comparison, very little immunoreactivity was observed with immature secretory granules or with the Golgi apparatus. No binding to secretory granules or mitochondria was observed with normal mouse serum or with unrelated sera. On Western blots, HSP60 antibody specifically interacted with a single 62 kDa islet cell protein. These results suggest the existence of an HSP60-related protein with a novel location in mature secretory granules of Beta cells. The preferential association of the HSP60-related protein with the insulin core was gradually lost in Beta cells of pre-diabetic non-obese diabetic mice, and correlated with the progression of insulitis. The decrease in the granular binding of the HSP60 antibody was accompanied by an increase in cytoplasm staining, and was concomitant with a significant expansion of the insulin core diameter. The altered distribution of the HSP60-related protein in prediabetic mice, together with our observation that immature secretory granules accumulate in these animals indicate that the presence of HSP60-related protein in secretory granules might be associated with the secretory function of Beta cells.
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