ArticleTissue engineering
Noninvasive in situ evaluation of osteogenic differentiation by time-resolved laser-induced fluorescence spectroscopy.
Article in Tissue engineering. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Current concepts and future of noninvasive procedures for diagnosing oral squamous cell carcinoma--a systematic review.Head & face medicine · 2015Pooled it
- Collagen: quantification, biomechanics, and role of minor subtypes in cartilage.Nature reviews. Materials · 2020Article
- Real-time multispectral fluorescence lifetime imaging using Single Photon Avalanche Diode arrays.Scientific reports · 2020Article
- Multimodal Label-Free Imaging for Detecting Maturation of Engineered Osteogenic Grafts.ACS biomaterials science & engineering · 2019Article
- Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs.Journal of biophotonics · 2018Article
- Noninvasive Optical Assessment of Implanted Engineered Tissues Correlates with Cytokine Secretion.Tissue engineering. Part C, Methods · 2018Article
- Detection of Pentosidine Cross-Links in Cell-Secreted Decellularized Matrices Using Time Resolved Fluorescence Spectroscopy.ACS biomaterials science & engineering · 2017Article
- Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization.Scientific reports · 2017Article
- Fluorescent lifetime imaging microscopy using Europium complexes improves atherosclerotic plaques discrimination.The international journal of cardiovascular imaging · 2016Article
- Application of time-resolved autofluorescence to label-free in vivo optical mapping of changes in tissue matrix and metabolism associated with myocardial infarction and heart failure.Biomedical optics express · 2015Article
- Nondestructive evaluation of tissue engineered articular cartilage using time-resolved fluorescence spectroscopy and ultrasound backscatter microscopy.Tissue engineering. Part C, Methods · 2012Article
- Fluorescence lifetime techniques in medical applications.Annals of biomedical engineering · 2012Review
- Noninvasive multimodal evaluation of bioengineered cartilage constructs combining time-resolved fluorescence and ultrasound imaging.Tissue engineering. Part C, Methods · 2011Article
- Optical spectroscopy and imaging for the noninvasive evaluation of engineered tissues.Tissue engineering. Part B, Reviews · 2008Review
- Line-scanning microscopy for time-gated and spectrally resolved fluorescence imaging.Journal of biological physics · 2008Article
- Fluorescence lifetime in cardiovascular diagnostics.Journal of biomedical opticsReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix (ECM) produced by putative stem cells (PLA cells) derived from human adipose tissue. We show that this optical spectroscopy technique can assess the relative expression of collagens (types I, III, IV, and V) within newly forming osteogenic ECM. The results are consistent with those obtained by conventional histochemical techniques (immunofluorescence and Western blot) and demonstrate that TR-LIFS is a potential tool for monitoring the expression of distinct collagen types and the formation of collagen cross-links in intact tissue constructs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.