ArticleGene therapy2004
Complete reversal of multidrug resistance by stable expression of small interfering RNAs targeting MDR1.
Article in Gene therapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 95 citations in OpenAlex.
- Importance of ABC Transporters in the Survival of Parasitic Nematodes and the Prospect for the Development of Novel Control Strategies.Pathogens (Basel, Switzerland) · 2023Review
- Interfering with the Expression of Ubiquitin-Like with PHD and Ring Finger Domains 1 Can Inhibit the Invasion of Human Renal Cell Carcinoma.Computational and mathematical methods in medicine · 2022Article
- Oncolytic virotherapy reverses chemoresistance in osteosarcoma by suppressing MDR1 expression.Cancer chemotherapy and pharmacology · 2021Article
- Exosomal microRNAs derived from mesenchymal stem cells: cell-to-cell messages.Cell communication and signaling : CCS · 2020Review
- Reversal of Multidrug Resistance in an Epirubicin-Resistant Gastric Cancer Cell SublineAsian Pacific journal of cancer prevention : APJCP · 2018Article
- Biophysics of cell membrane lipids in cancer drug resistance: Implications for drug transport and drug delivery with nanoparticles.Advanced drug delivery reviews · 2013Review
- Combination of siRNA-directed Gene Silencing With Cisplatin Reverses Drug Resistance in Human Non-small Cell Lung Cancer.Molecular therapy. Nucleic acids · 2013Article
- siRNA-mediated down-regulation of P-glycoprotein in a Xenograft tumor model in NOD-SCID mice.Pharmaceutical research · 2011Article
- Personalized cancer approach: using RNA interference technology.World journal of surgery · 2011Review
- The use of nanoparticle-mediated targeted gene silencing and drug delivery to overcome tumor drug resistance.Biomaterials · 2010Article
- Reversal of multi-drug resistance by pSUPER-shRNA-mdr1 in vivo and in vitro.World journal of gastroenterology · 2009Article
- Role of the highly structured 5'-end region of MDR1 mRNA in P-glycoprotein expression.The Biochemical journal · 2007Article
- RNAi-mediated knockdown of target genes: a promising strategy for pancreatic cancer research.Cancer gene therapy · 2007Review
- Reversing multidrug resistance by RNA interference through the suppression of MDR1 gene in human hepatoma cells.World journal of gastroenterology · 2006Article
- Prospects of RNA interference therapy for cancer.Gene therapy · 2006Review
- Reversal of adriamycin resistance in human mammary cancer cells by small interfering RNA of MDR1 and MDR3 genes.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2006Article
- The therapeutic potential of RNA interference.FEBS letters · 2005Review
- RNA interference and potential therapeutic applications of short interfering RNAs.Cancer gene therapy · 2005Review
- The role of small RNAs in human diseases: potential troublemaker and therapeutic tools.Medicinal research reviews · 2005Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Overexpression of P-glycoprotein, encoded by the MDR1 gene, confers multidrug resistance (MDR) on cancer cells and is a frequent impediment to successful chemotherapy. Recent developments in the use of small interfering RNAs to inhibit specific protein expression have highlighted their potential use as therapeutic agents. We have expressed two different short hairpin RNAs from stably integrated plasmids in doxorubicin-resistant K562 leukaemic cells. The MDR1-targeted RNA interference (RNAi) resulted in decreased MDR1 mRNA, abolished P-glycoprotein expression, and completely reversed the MDR phenotype to that of the drug-sensitive K562 parental line. This study demonstrates that MDR, which is solely due to overexpression of P-glycoprotein, can be reversed by RNAi. These target sequences can in the future be integrated into gene therapy vectors with potential clinical application.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.