ArticleWorld journal of gastroenterology2004
Association of differentially expressed genes with activation of mouse hepatic stellate cells by high-density cDNA microarray.
Article in World journal of gastroenterology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
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- Stiffness is associated with hepatic stellate cell heterogeneity during liver fibrosis.American journal of physiology. Gastrointestinal and liver physiology · 2022Article
- Inhibition of vascular endothelial growth factor signaling facilitates liver repair from acute ethanol-induced injury in zebrafish.Disease models & mechanisms · 2016Article
- Employment of gene expression profiling to identify transcriptional regulators of hepatic stellate cells.Fibrogenesis & tissue repair · 2012Article
- Daily genetic profiling indicates JAK/STAT signaling promotes early hepatic stellate cell transdifferentiation.World journal of gastroenterology · 2010Article
- Toxicogenomic biomarkers for liver toxicity.Journal of toxicologic pathology · 2009Article
- Hepatic stellate cells: protean, multifunctional, and enigmatic cells of the liver.Physiological reviews · 2008Review
- The hepatic transcriptome in human liver disease.Comparative hepatology · 2006Article
- Dynamic expression of extracellular signal-regulated kinase in rat liver tissue during hepatic fibrogenesis.World journal of gastroenterology · 2006Article
- Effect of Chinese medicine Qinggan Huoxuefang on inducing HSC apoptosis in alcoholic liver fibrosis rats.World journal of gastroenterology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo characterize the gene expression profiles associated with activation of mouse hepatic stellate cell (HSC) and provide novel insights into the pathogenesis of hepatic fibrosis.
methodsMice HSCs were isolated from BALB/c mice by in situ perfusion of collagenase and pronase and single-step density Nycodenz gradient. Total RNA and mRNA of quiescent HSC and culture-activated HSC were extracted, quantified and reversely transcribed into cDNA. cDNAs from activated HSC were labeled with Cy5 and cDNAs from the quiescent HSC were labeled with Cy3, which were mixed with equal quantity, then hybridized with cDNA chips containing 4000 genes. Chips were washed, scanned and analyzed. Increased expression of 4 genes and decreased expression of one gene in activated HSC were confirmed by reverse transcription-polymerase chain reaction (RT-PCR).
resultsA total of 835 differentially expressed genes were identified by cDNA chip between activated and quiescent HSC, and 465 genes were highly expressed in activated HSC. The differentially expressed genes included those involved in protein synthesis, cell-cycle regulation, apoptosis, and DNA damage response.
conclusionMany genes implicated in intrahepatic inflammation, fibrosis and proliferation were up-regulated in activated HSC. cDNA microarray is an effective technique in screening for differentially expressed genes between two different situations of the HSC. Further analysis of the obtained genes will help understand the molecular mechanism of activation of HSC and hepatic fibrosis.
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