ArticleJournal of muscle research and cell motility2004
Contractile protein concentrations in human single muscle fibers.
Article in Journal of muscle research and cell motility, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Effects of ageing on single muscle fibre contractile function following short-term immobilisation.The Journal of physiology · 2011Trial
- Human skeletal muscle structure and function preserved by vibration muscle exercise following 55 days of bed rest.European journal of applied physiology · 2006Trial
- Dietary linoleic acid, antioxidants, and flight training influence the activity of oxidative enzymes in European Starlings (Sturnus vulgaris).Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2021Article
- Prostaglandin E2/cyclooxygenase pathway in human skeletal muscle: influence of muscle fiber type and age.Journal of applied physiology (Bethesda, Md. : 1985) · 2016Article
- Aerobic exercise training induces skeletal muscle hypertrophy and age-dependent adaptations in myofiber function in young and older men.Journal of applied physiology (Bethesda, Md. : 1985) · 2012Article
- A new method to study in vivo protein synthesis in slow- and fast-twitch muscle fibers and initial measurements in humans.Journal of applied physiology (Bethesda, Md. : 1985) · 2010Article
- Influence of aging and long-term unloading on the structure and function of human skeletal muscle.Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2009Article
- Release of alpha-actin into serum after skeletal muscle damage.British journal of sports medicine · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The intent of this investigation was twofold: (1) to develop a convenient method for analyzing skeletal muscle protein concentrations in a large number of individual human single fibers and (2) to compare the myosin heavy chain (MHC) and actin concentrations in fibers expressing pure MHC I or MHC IIa. Individual vastus lateralis fibers were dissected from five individuals (3 M, 2 F; 24 +/- 1 years) and used to determine single fiber total protein (TP) concentration and MHC distribution. Fibers expressing pure MHC I and MHC IIa were further analyzed for MHC (252 fibers; mean of 50/subject) and actin (160 fibers; mean of 32/subject) concentration relative to TP. Single fiber MHC concentration was 26 +/- 4% greater (P < 0.05) in MHC IIa (364 +/- 39 micrograms MHC/mg TP) vs. MHC I (266 +/- 29 micrograms MHC/mg TP) fibers. No differences (P > 0.05) were noted in single fiber actin concentration (MHC I: 171 +/- 17 micrograms actin/mg TP; MHC IIa: 165 +/- 17 micrograms actin/mg TP). These data indicate that within the TP fraction, skeletal muscle fibers contain differing amounts of MHC, and this appears to be fiber type specific. These data and methods have implications for the study of human muscle fiber type specific alterations in various protein concentrations in response to exercise, models of unloading, and aging.
Indexed as
Identifiers
15160488What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.