ArticleJournal of muscle research and cell motility2004
Does the myosin V neck region act as a lever?
Article in Journal of muscle research and cell motility, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Kinetic signatures of myosin-5B, the motor involved in microvillus inclusion disease.The Journal of biological chemistry · 2017Article
- Robust mechanobiological behavior emerges in heterogeneous myosin systems.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Omecamtiv Mecarbil Enhances the Duty Ratio of Human β-Cardiac Myosin Resulting in Increased Calcium Sensitivity and Slowed Force Development in Cardiac Muscle.The Journal of biological chemistry · 2017Article
- Myosin regulatory light chain phosphorylation enhances cardiac β-myosin in vitro motility under load.Archives of biochemistry and biophysics · 2015Article
- Flexural Stiffness of Myosin Va Subdomains as Measured from Tethered Particle Motion.Journal of biophysics (Hindawi Publishing Corporation : Online) · 2015Article
- Impact of familial hypertrophic cardiomyopathy-linked mutations in the NH2 terminus of the RLC on β-myosin cross-bridge mechanics.Journal of applied physiology (Bethesda, Md. : 1985) · 2014Article
- A Toxoplasma gondii class XIV myosin, expressed in Sf9 cells with a parasite co-chaperone, requires two light chains for fast motility.The Journal of biological chemistry · 2014Article
- Magnesium modulates actin binding and ADP release in myosin motors.The Journal of biological chemistry · 2014Article
- Design principles governing the motility of myosin V.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Understanding cardiomyopathy phenotypes based on the functional impact of mutations in the myosin motor.Circulation research · 2012Review
- Lever-arm mechanics of processive myosins.Biophysical journal · 2011Review
- Cardiomyopathy-linked myosin regulatory light chain mutations disrupt myosin strain-dependent biochemistry.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Structured post-IQ domain governs selectivity of myosin X for fascin-actin bundles.The Journal of biological chemistry · 2010Article
- The lever arm effects a mechanical asymmetry of the myosin-V-actin bond.Biophysical journal · 2010Article
- Removal of the cardiac myosin regulatory light chain increases isometric force production.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Coarse-Grained Structural Modeling of Molecular Motors Using Multibody Dynamics.Cellular and molecular bioengineering · 2009Article
- Essential features of the class V myosin from budding yeast for ASH1 mRNA transport.Molecular biology of the cell · 2009Article
- Head-neck domain of Arabidopsis myosin XI, MYA2, fused with GFP produces F-actin patterns that coincide with fast organelle streaming in different plant cells.BMC plant biology · 2008Article
- Load and Pi control flux through the branched kinetic cycle of myosin V.The Journal of biological chemistry · 2008Article
- Cargo-binding makes a wild-type single-headed myosin-VI move processively.Biophysical journal · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Recent research efforts from several groups have addressed the question of whether the amplitude of myosin's unitary step size is proportional to the length of the neck region. Unconventional myosin V, which has an extended neck region with 6IQ motifs, provides a natural template by which to test the lever arm model via mutational analysis. The most stringent test requires that a series of single-headed molecules from the same myosin class be analyzed. Here we characterized the unitary mechanics of three single-headed fragments of myosin V expressed in the baculovirus/insect cell system. Each construct consisted of the motor domain (MD) and a variable number of IQ motifs (MD2IQ, MD4IQ and MD6IQ) that bind calmodulin, followed by an epitope tag so that the molecule can be attached to the nitrocellulose surface via an antibody. The results show a correlation between the unitary step size and the number of IQ motifs, confirming that the myosin neck region acts as a lever. The step size of MD2IQ is twice that observed from single-headed subfragments of class II myosins with the same neck length. Our results are discussed in relation to data obtained concurrently from other laboratories with similar constructs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.