ArticlePlant molecular biology2004
Cloning and functional analyses of a gene from sugar beet up-regulated upon cyst nematode infection.
Article in Plant molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- Development of a Species-Specific SCAR-PCR Assay for Direct Detection of Sugar Beet Cyst Nematode (Life (Basel, Switzerland) · 2021Article
- Rapid and Visual Detection ofInternational journal of molecular sciences · 2021Article
- Molecular insights into the compatible and incompatible interactions between sugar beet and the beet cyst nematode.BMC plant biology · 2020Article
- Differential gene expression and associated QTL mapping for cotton yield based on a cDNA-AFLP transcriptome map in an immortalized F2.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Phenotypic and molecular evaluation of cotton hairy roots as a model system for studying nematode resistance.Plant cell reports · 2009Article
- Differential gene expression of related wheat lines with contrasting levels of head blight resistance after Fusarium graminearum inoculation.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Construction of a potato transcriptome map based on the cDNA-AFLP technique.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- A complete physical map of a wild beet (Beta procumbens) translocation in sugar beet.Molecular genetics and genomics : MGG · 2006Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cDNA-AFLP technique was used to isolate sugar beet genes up-regulated upon infection with the beet cyst nematode Heterodera schachtii. Hairy root cultures were obtained from resistant plants carrying a Beta procumbens translocation as well as from a non-resistant control. mRNA was isolated from hairy root clones and sugar beet plants infected or not with the beet cyst nematode and 8000 transcript-derived fragments (TDFs) were analysed. One TDF was found to be differentially expressed in both materials and was further investigated. Real-time PCR confirmed that this TDF is specifically up-regulated in resistant sugar beet upon nematode infection and its full-length cDNA was isolated. Sequence analysis suggests that the gene encodes a 317 amino acid polypeptide of unknown function. No homology to any sequence present in the public databases could be detected. To further elucidate its function in resistance to the beet cyst nematode, the cDNA was transformed into hairy roots of susceptible sugar beet under the control of the 35S promoter and hairy root clones were inoculated with nematodes. The number of developing females was significantly reduced in 12 out of 15 clones resulting from independent transgenic events suggesting that the gene can be used for inducing cyst nematode resistance in plants.
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