ArticlePlant molecular biology2004
Over-expression of a scopoletin glucosyltransferase in Nicotiana tabacum leads to precocious lesion formation during the hypersensitive response to tobacco mosaic virus but does not affect virus resistance.
Article in Plant molecular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 75 citations in OpenAlex.
- Proteomic Profiling ofInternational journal of molecular sciences · 2024Article
- Transcriptional insights of citrus defense response against Diaporthe citri.BMC plant biology · 2023Article
- Subfunctionalization of a monolignol to a phytoalexin glucosyltransferase is accompanied by substrate inhibition.Plant communications · 2023Article
- Review
- Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response toLife (Basel, Switzerland) · 2022Article
- Tomato leaves under stress: a comparison of stress response to mild abiotic stress between a cultivated and a wild tomato species.Plant molecular biology · 2021Article
- Consequences of LED Lights on Root Morphological Traits and Compounds Accumulation inInternational journal of molecular sciences · 2021Article
- Phenylpropanoid Pathway Engineering: An Emerging Approach towards Plant Defense.Pathogens (Basel, Switzerland) · 2020Review
- Identification of the key genes involved in the regulation of symbiotic pathways induced by3 Biotech · 2020Article
- You Want it Sweeter: How Glycosylation Affects Plant Response to Oxidative Stress.Frontiers in plant science · 2020Review
- Tomato glycosyltransferase Twi1 plays a role in flavonoid glycosylation and defence against virus.BMC plant biology · 2019Article
- Article
- Deciphering the role of phytoalexins in plant-microorganism interactions and human health.Molecules (Basel, Switzerland) · 2014Review
- Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling.Journal of experimental botany · 2014Article
- 2-Oxoglutarate-dependent dioxygenases in the biosynthesis of simple coumarins.Frontiers in plant science · 2014Review
- Modulation of phytoalexin biosynthesis in engineered plants for disease resistance.International journal of molecular sciences · 2013Review
- The pepper E3 ubiquitin ligase RING1 gene, CaRING1, is required for cell death and the salicylic acid-dependent defense response.Plant physiology · 2011Article
- Genome-wide analysis of phenylpropanoid defence pathways.Molecular plant pathology · 2010Review
- Molecular cloning and characterization of a novel tomato xylosyltransferase specific for gentisic acid.Journal of experimental botany · 2010Article
- Streptomyces scabiei and its toxin thaxtomin A induce scopoletin biosynthesis in tobacco and Arabidopsis thaliana.Plant cell reports · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotiana tabacum Togt encodes a scopoletin glucosyltransferase (UDPglucose:scopoletin O -beta-D-glucosyltrans- ferase, EC 2.4.1.128) known to act in vitro on many different substrates including the 6-methoxy-7-hydroxy- coumarin scopoletin. This phenolic compound accumulates in vast amounts, essentially in its glucosylated form scopolin, in tobacco during the hypersensitive response (HR) to tobacco mosaic virus (TMV). To identify the physiological role of this pathogen-inducible UDP-Glc glucosyltransferase (UGT), we generated TOGT over-expressing transgenic plants. Although no endogenous scopoletin or scopolin could be detected before infection, the accumulation of both the aglycone and the glucoside was found to be 2-fold higher in transgenic plants after inoculation with TMV than in wild-type plants. Scopoletin UGT activity in plants over-expressing Togt was significantly higher during the HR than in control plants. This up-regulated activity was associated with a strong increase of the bright blue fluorescence surrounding the HR-necrotic lesions under UV light, which is known to correlate with scopoletin and scopolin abundance. Necrosis appeared sooner in transgenic plants and lesions developed faster, suggesting an accelerated HR. Unexpectedly, the viral content in each lesion was not significantly different in transgenic and in wild-type plants. These results are discussed in relation to the role of TOGT as the major UDP-Glc: scopoletin glucosyltransferase and to the importance of scopoletin accumulation during the HR.
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