ArticleTheriogenology2004
Initial studies on sperm cryopreservation of a live-bearing fish, the green swordtail Xiphophorus helleri.
Article in Theriogenology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Transitioning from a research protocol to a scalable applied pathway for Xenopus laevis sperm cryopreservation at a national stock center: The effect of cryoprotectants.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Article
- Optimization of Sperm Management and Fertilization in Zebrafish (Animals : an open access journal from MDPI · 2021Article
- Development of germplasm repositories to assist conservation of endangered fishes: Examples from small-bodied livebearing fishes.Theriogenology · 2019Article
- Production of live young with cryopreserved sperm from the endangered livebearing fish Redtail Splitfin (Xenotoca eiseni, Rutter, 1896).Animal reproduction science · 2018Article
- Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, Xenotoca eiseni.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2018Article
- Vitrification as an Alternative Approach for Sperm Cryopreservation in Marine Fishes.North American journal of aquaculture · 2017Article
- The effects of osmolality on sperm quality in Jenynsia multidentata (Cyprinodontiformes: Anablepidae).Fish physiology and biochemistry · 2016Article
- Article
- Offspring production with cryopreserved sperm from a live-bearing fish Xiphophorus maculatus and implications for female fecundity.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2012Article
- Production of F₁ offspring with vitrified sperm from a live-bearing fish, the green swordtail Xiphophorus hellerii.Zebrafish · 2011Article
- Zebrafish reproduction: revisiting in vitro fertilization to increase sperm cryopreservation success.PloS one · 2011Article
- Sperm motility initiation and duration in a euryhaline fish, medaka (Oryzias latipes).Theriogenology · 2009Article
- Current status of sperm cryopreservation in biomedical research fish models: zebrafish, medaka, and Xiphophorus.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2009Review
- Sperm cryopreservation of a live-bearing fish, Xiphophorus couchianus: male-to-male variation in post-thaw motility and production of F(1) hybrid offspring.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2009Article
- Biophysics of zebrafish (Danio rerio) sperm.Cryobiology · 2009Article
- Production of F1 interspecies hybrid offspring with cryopreserved sperm from a live-bearing fish, the swordtail Xiphophorus helleri.Biology of reproduction · 2007Article
- Post-thaw amendment of cryopreserved sperm for use in artificial insemination of a viviparous fish, the green swordtailAquaculture (Amsterdam, Netherlands) · 2006Article
- Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail Xiphophorus helleri.Cryobiology · 2006Article
- A theoretically estimated optimal cooling rate for the cryopreservation of sperm cells from a live-bearing fish, the green swordtail Xiphophorus helleri.Theriogenology · 2005Article
- Subzero water permeability parameters and optimal freezing rates for sperm cells of the southern platyfish, Xiphophorus maculatus.Cryobiology · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Swordtails and platyfish of the genus Xiphophorus are valuable models for biomedical research and are also commercially raised as ornamental fish valued by aquarists. While research use and commercial interest increases yearly in these fish, cryopreservation of sperm is unexplored in this genus. Xiphophorus are live-bearing fishes characterized by small body sizes, limited sperm volumes, and internal fertilization, an atypical reproductive mode for fish. These attributes make research involving cryopreservation of Xiphophorus germplasm challenging. To explore methods for sperm cryopreservation, this study evaluated the effect of different loading volumes of sperm suspension in 0.25-ml French straws, different dilution ratios of sperm to extender, an osmolality range of extender without cryoprotectant and with dimethyl sulfoxide (DMSO) as cryoprotectant, and short-term storage at room temperature and 4 degrees C after thawing. No significant difference in sperm motility due to straw loading volume was observed after thawing. Sperm motility was observed to decrease with increasing dilution. The osmolality of Hanks' balanced salt solution (HBSS) without cryoprotectant in which the highest sperm motility (67%) was observed was 320 +/- 3 mOsm/kg, which was also the osmolality of X. helleri blood plasma. When cryopreserved with 10% DMSO, however, the highest motilities within 10 min after thawing were observed with HBSS in the range of 240-300 mOsm/kg. Sperm suspended in HBSS at 320 mOsm/kg with a dilution factor of 100 maintained motility for 24h at room temperature, but persisted for 10 days when stored at 4 degrees C. These results provided the first evidence that cryopreservation may be applied to conservation of genetic resources in live-bearing fishes.
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