ArticleMethods (San Diego, Calif.)2004
In vivo labeling of fission yeast DNA with thymidine and thymidine analogs.
Article in Methods (San Diego, Calif.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 72 citations in OpenAlex.
- A Method to Calibrate Chemical-Agnostic Quantitative Adverse Outcome Pathways on Multiple Chemical Data.Chemical research in toxicology · 2026Article
- Checkpoint-Dependent Sensitivities to Nucleoside Analogues Uncover Specific Patterns of Genomic Instability.Current issues in molecular biology · 2025Article
- Mrc1 regulates parental histone segregation and heterochromatin inheritance.Molecular cell · 2024Article
- Coordination of histone chaperones for parental histone segregation and epigenetic inheritance.Genes & development · 2024Article
- The cell cycle and cell size influence the rates of global cellular translation and transcription in fission yeast.The EMBO journal · 2023Article
- The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stability.Cell reports · 2023Article
- Cosegregation of asymmetric features during cell division.Open biology · 2021Article
- Robust and flexible platform for directed evolution of yeast genetic switches.Nature communications · 2021Article
- Mre11 exonuclease activity removes the chain-terminating nucleoside analog gemcitabine from the nascent strand during DNA replication.Science advances · 2020Article
- Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.Nature communications · 2019Article
- Evidence for Compartmentalized Axonal Mitochondrial Biogenesis: Mitochondrial DNA Replication Increases in Distal Axons As an Early Response to Parkinson's Disease-Relevant Stress.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- A Tool for the Import of Natural and Unnatural Nucleoside Triphosphates into Bacteria.Journal of the American Chemical Society · 2018Article
- An estradiol-inducible promoter enables fast, graduated control of gene expression in fission yeast.Yeast (Chichester, England) · 2017Article
- Article
- Establishment of a promoter-based chromatin architecture on recently replicated DNA can accommodate variable inter-nucleosome spacing.Nucleic acids research · 2016Article
- Accurate concentration control of mitochondria and nucleoids.Science (New York, N.Y.) · 2016Article
- A markerless gene deletion and integration system for Thermoanaerobacter ethanolicus.Biotechnology for biofuels · 2016Article
- Article
- A Tool for Multiple Targeted Genome Deletions that Is Precise, Scar-Free, and Suitable for Automation.PloS one · 2015Article
- Incorporation of thymidine analogs for studying replication kinetics in fission yeast.Methods in molecular biology (Clifton, N.J.) · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In vivo labeling of DNA with thymidine and thymidine analogs has long been a cornerstone of replication studies. Unfortunately, yeast lack a thymidine salvage pathway and thus do not incorporate exogenous thymidine. Specifically, yeast neither efficiently take up exogenous thymidine from their growth media nor phosphorylate it to thymidylate, the precursor of dTTP. We have overcome these problems in fission yeast by expressing the human equilibrative nucleoside transporter 1 (hENT1) along with herpes simplex virus thymidine kinase (tk). hENT1 tk cells are healthy and efficiently incorporate exogenous thymidine and thymidine analogs. We present protocols for labeling DNA with tritiated thymidine, for in situ detection of incorporated BrdU by immunofluorescence, for double labeling with CldU and IdU, for CsCl gradient separation of IdU-labeled DNA, and for using hENT1 and tk as both positive and negative selection markers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.