ArticleCryobiology2004
Sperm cryopreservation of green swordtail Xiphophorus helleri, a fish with internal fertilization.
Article in Cryobiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- Cryopreservation of Roughscale Sole (Animals : an open access journal from MDPI · 2022Article
- The Need for a Framework Addressing the Temporal Aspects of Fish Sperm Motility Leading to Community-Level Standardization.Zebrafish · 2022Article
- Production of live young with cryopreserved sperm from the endangered livebearing fish Redtail Splitfin (Xenotoca eiseni, Rutter, 1896).Animal reproduction science · 2018Article
- Cryopreservation of sperm bundles (spermatozeugmata) from endangered livebearing goodeids.Cryobiology · 2018Article
- Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, Xenotoca eiseni.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2018Article
- The effects of osmolality on sperm quality in Jenynsia multidentata (Cyprinodontiformes: Anablepidae).Fish physiology and biochemistry · 2016Article
- Article
- Production of F₁ offspring with vitrified sperm from a live-bearing fish, the green swordtail Xiphophorus hellerii.Zebrafish · 2011Article
- Outlook for development of high-throughput cryopreservation for small-bodied biomedical model fishes.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2011Article
- Zebrafish reproduction: revisiting in vitro fertilization to increase sperm cryopreservation success.PloS one · 2011Article
- Evaluation of cryoprotectant and cooling rate for sperm cryopreservation in the euryhaline fish medaka Oryzias latipes.Cryobiology · 2010Article
- Current status of sperm cryopreservation in biomedical research fish models: zebrafish, medaka, and Xiphophorus.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2009Review
- Sperm cryopreservation of a live-bearing fish, Xiphophorus couchianus: male-to-male variation in post-thaw motility and production of F(1) hybrid offspring.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2009Article
- Biophysics of zebrafish (Danio rerio) sperm.Cryobiology · 2009Article
- Production of F1 interspecies hybrid offspring with cryopreserved sperm from a live-bearing fish, the swordtail Xiphophorus helleri.Biology of reproduction · 2007Article
- Post-thaw amendment of cryopreserved sperm for use in artificial insemination of a viviparous fish, the green swordtailAquaculture (Amsterdam, Netherlands) · 2006Article
- Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail Xiphophorus helleri.Cryobiology · 2006Article
- A theoretically estimated optimal cooling rate for the cryopreservation of sperm cells from a live-bearing fish, the green swordtail Xiphophorus helleri.Theriogenology · 2005Article
- Subzero water permeability parameters and optimal freezing rates for sperm cells of the southern platyfish, Xiphophorus maculatus.Cryobiology · 2005Article
- Sperm cryopreservation of a live-bearing fish, the platyfish Xiphophorus couchianus.Theriogenology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Sperm cryopreservation for fishes with internal fertilization is essentially unexplored although many species of these fishes are valuable biomedical research models. To explore methods for sperm cryopreservation within the live-bearing genus Xiphophorus, this study used X. helleri to evaluate the effects of cryoprotectant, osmotic pressure, cooling rate, equilibration time, and sperm-to-extender ratio. Sperm motility and survival duration after thawing showed significant differences among different cryoprotectants with the highest motility at 10 min after thawing obtained with 14% glycerol. With subsequent use of 14% glycerol as the cryoprotectant, the highest motility after thawing was observed with Hanks' balanced salt solution (HBSS) at 300 mOsmol/kg. Samples cooled from 5 to -80 degrees C at 20 degrees C/min yielded the highest post-thaw motility although no significant difference was found in the first 4h after thawing for cooling rates across the range of 20-35 degrees C/min. Evaluation of equilibration time revealed no significant difference between 20 min and 2h, but the highest motility at 10 min after thawing was found with a 20-min equilibration. Dilution ratios of sperm-to-extender at 1:20, 1:60, and 1:120 showed no significant differences in motility and survival duration after thawing, but the dilution of sperm solutions with HBSS (320 mOsmol/kg) immediately after thawing reduced the decline of sperm motility, and significantly prolonged the survival duration. Based on these findings, the highest average sperm motility (77%) at 10 min after thawing was obtained when sperm were suspended in HBSS at 300 mOsmol/kg with 14% glycerol as cryoprotectant, diluted at a ratio of sperm to HBSS-glycerol of 1:20, equilibrated for 10 min, cooled at 20 degrees C/min from 5 to -80 degrees C before being plunged in liquid nitrogen, and thawed in a 40 degrees C water bath for 7s. If diluted immediately after thawing, sperm frozen by the protocol above retained continuous motility after thawing for more than 8 days when stored at 4 degrees C.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.