ArticleNucleic acids research2004
A protein-dependent riboswitch controlling ptsGHI operon expression in Bacillus subtilis: RNA structure rather than sequence provides interaction specificity.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 32 citations in OpenAlex.
- A New Mechanism of Carbon Metabolism and Acetic Acid Balance Regulated by CcpA.Microorganisms · 2023Article
- Lipid membranes modulate the activity of RNA through sequence-dependent interactions.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Moonlighting inMicroorganisms · 2021Review
- The computational approaches of lncRNA identification based on coding potential:Computational and structural biotechnology journal · 2020Review
- Competitive folding of anti-terminator/terminator hairpins monitored by single molecule FRET.Nucleic acids research · 2013Article
- Determinants of interaction specificity of the Bacillus subtilis GlcT antitermination protein: functionality and phosphorylation specificity depend on the arrangement of the regulatory domains.The Journal of biological chemistry · 2012Article
- Identification of microRNA precursors based on random forest with network-level representation method of stem-loop structure.BMC bioinformatics · 2011Article
- Prevention of cross-talk in conserved regulatory systems: identification of specificity determinants in RNA-binding anti-termination proteins of the BglG family.Nucleic acids research · 2011Article
- An overview of RNAs with regulatory functions in gram-positive bacteria.Cellular and molecular life sciences : CMLS · 2010Review
- Identification and classification of ncRNA molecules using graph properties.Nucleic acids research · 2009Article
- Article
- An in silico analysis of T-box regulated genes and T-box evolution in prokaryotes, with emphasis on prediction of substrate specificity of transporters.BMC genomics · 2008Article
- The DeoR-type regulator SugR represses expression of ptsG in Corynebacterium glutamicum.Journal of bacteriology · 2007Article
- Complete probabilistic analysis of RNA shapes.BMC biology · 2006Article
- Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples.Nucleic acids research · 2006Article
- Multiple-mutation reaction: a method for simultaneous introduction of multiple mutations into the glpK gene of Mycoplasma pneumoniae.Applied and environmental microbiology · 2005Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The Gram-positive soil bacterium Bacillus subtilis transports glucose by the phosphotransferase system. The genes for this system are encoded in the ptsGHI operon. The expression of this operon is controlled at the level of transcript elongation by a protein-dependent riboswitch. In the absence of glucose a transcriptional terminator prevents elongation into the structural genes. In the presence of glucose, the GlcT protein is activated and binds and stabilizes an alternative RNA structure that overlaps the terminator and prevents termination. In this work, we have studied the structural and sequence requirements for the two mutually exclusive RNA structures, the terminator and the RNA antiterminator (the RAT sequence). In both cases, the structure seems to be more important than the actual sequence. The number of paired and unpaired bases in the RAT sequence is essential for recognition by the antiterminator protein GlcT. In contrast, mutations of individual bases are well tolerated as long as the general structure of the RAT is not impaired. The introduction of one additional base in the RAT changed its structure and resulted in complete loss of interaction with GlcT. In contrast, this mutant RAT was efficiently recognized by a different B.subtilis antitermination protein, LicT.
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